Molecular genetic studis on the construction of pollen surface structures and their function for pollination

2014 Fiscal Year Final Research Report

• Project/Area Number: 23370018
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nagoya University
• Principal Investigator: ISHIGURO Sumie 名古屋大学, 生命農学研究科, 准教授 (50260039)
• Co-Investigator(Renkei-kenkyūsha): SUZUKI Takamasa 中部大学, 応用生物学部, 講師 (50535797)
• Research Collaborator: SUZUKI Toshiya 名古屋大学, 大学院生命農学研究科, 研究員
• Project Period (FY): 2011-04-01 – 2015-03-31
• Keywords: 植物 / 生殖 / 花粉 / 細胞壁 / イメージング / 高速シーケンサー

Outline of Final Research Achievements

Exine, a rigid and chemically stable architecture on the surface of pollen grains, serves a protector of male gametes. We identified many Arabidopsis kaonashi mutants that produce pollen grains with abnormal exine structure. Twelve responsible genes of the mutants determined in this study revealed that a certain class of polysaccharides plays important roles for exine construction. Pollen coat consisting mainly of proteins and lipids is another surface component of pollen grains. Biosynthesis and requirement of two major proteins, EXL and GRP, in Arabidopsis pollen coat were analyzed. We also visualized the process of pollen coat formation in anthers and its behavior on the surface of stigmatic papilla cells.

Free Research Field

植物分子遺伝学