2013 Fiscal Year Final Research Report
Analysis of auxin-induced activation of the plasma membrane H+-ATPase
| Project/Area Number |
23370019
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Nagoya University |
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Principal Investigator |
KINOSHITA Toshinori 名古屋大学, トランスフォーマティブ生命分子研究所(理), 教授 (50271101)
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| Co-Investigator(Renkei-kenkyūsha) |
TAKAHASHI Koji 名古屋大学, 大学院・理学研究科, 助教 (40283379)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | オーキシン / 細胞膜プロトンポンプ / オーキシン受容体 / 胚軸伸長 |
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| Research Abstract |
In this study, we showed that auxin induces phosphorylation of the penultimate Thr in the plasma membrane H+-ATPase without involvement of the identified auxin receptors, TIR1/AFB, in Arabidopsis etiolated seedlings, and that auxin-induced phosphorylation is required for the early phase of auxin-induced hypocotyl elongation. Now, we are trying to identify the unidentified auxin receptor by reverse genetic and biochemical approaches. In addition, we have started to screen the mutants, which show reduced auxin-induced hypocotyl elongation and phosphorylation of the plasma membrane H+-ATPase in EMS-treated Arabidopsis etiolated seedlings.
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[Journal Article] TWIN SISTER OF FT, GIGANTEA, and CONSTANS have a positive but indirect effect on blue light-induced stomatal opening in Arabidopsis thaliana2013
Author(s)
Ando E, Ohnishi M, Wang Y, Matsushita T, Watanabe A, Hayashi Y, Fujii M, Ma JF, Inoue S, Kinoshita T
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Journal Title
Plant Physiology
Volume: 162(3)
Pages: 1529-1538
DOI
Peer Reviewed
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[Journal Article] FLOWERING LOCUS T regulates stomatal opening2011
Author(s)
Kinoshita T, Ono N, Hayashi Y, Morimoto S, Nakamura S, Soda M, Kato Y, Ohnishi M, Nakano T, Inoue S, Shimazaki K
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Journal Title
Current Biology
Volume: 21
Pages: 1232-1238
DOI
Peer Reviewed
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