2013 Fiscal Year Final Research Report
Dynamic analyses of subcellular structures using pinpoint functional depletion
| Project/Area Number |
23370029
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphology/Structure
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| Research Institution | Tokyo University of Science |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KUTSUNA Natsumaro 東京大学, 大学院・新領域創成科学研究科, 助教 (70578559)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 細胞分裂 / 染色体 / 顕微鏡 / イメージング |
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| Research Abstract |
Dynamic analyses of subcellular structures using pinpoint functional depletion
The purpose of this project is to reveal the dynamics of cell division in animals and plants using pinpoint functional depletion. The pinpoint functional depletion using a chromosomal protein RBMX fused with a photosensitizer protein was successfully performed by high-power laser. Spatiotemporal inactivation of RBMX with the photosensitizer was performed in combination with live cell imaging. At the result, RBMX interaction with chromatin is turned out through the spatiotemporal inactivation. This method to induce the delay of cell division can be performed through generation of reactive oxygen species and structural destruction of the protein complex by laser irradiation to fluorescent protein-labelled subcellular structures.
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Research Products
(25 results)
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[Journal Article] The kinesin-like protein TOP promotes Aurora localization and induces mitochondrial, chloroplast and nuclear division2013
Author(s)
Yoshida, Y., Fujiwara, T., Imoto, Y., Yoshida, M., Ohnuma, M., Hirooka, S., Misumi, O., Kuroiwa, H. Kato, S., Matsunaga, S. and Kuroiwa, T.
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Journal Title
JOURNAL OF CELL SCIENCE
Volume: 126
Pages: 2392-2400
DOI
Peer Reviewed
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[Journal Article] New insights into the dynamics of plant cell nuclei and chromosomes2013
Author(s)
Matsunaga, S., Katagiri, Y., Nagashima, Y., Sugiyama, T., Hasegawa, J., Hayashi, K. and Sakamoto, T.
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Journal Title
INTERNATIONAL REVIEW OF CELL AND MOLECULAR BIOLOGY
Volume: 305
Pages: 253-301
DOI
Peer Reviewed
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[Journal Article] Cytokinins control endocycle onset by promoting the expression of an APC/C activator in Arabidopsis roots2013
Author(s)
Takahashi, N., Kajihara, T., Okamura, C., Kim, Y., Katagiri, Y., Okushima, Y., Matsunaga, S., Hwang, I., and Umeda, M.
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Journal Title
CURRENT BIOLOGY
Volume: 23
Pages: 1812-1817
DOI
Peer Reviewed
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[Journal Article] ASURA (Phb2) interacts with Scc1 through chromatin2013
Author(s)
Equilibrina, I., Matsunaga, S., Morimoto, A., Hashimoto, T., Uchiyama, S. and Fukui, K.
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Journal Title
CYTOGENETIC AND GENOME RESEARCH
Volume: 139
Pages: 225-233
DOI
Peer Reviewed
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[Journal Article] RBMX : a regulator for maintenance and centromeric protection of sister chromatid cohesion2012
Author(s)
Matsunaga, S., Takata, H., Morimoto, A., Hayashihara, K., Higashi, T., Akatsuchi, K., Mizusawa, E., Yamakawa, M., Ashida, M., Matsunaga, T. M., Azuma, T., Uchiyama, S. and Fukui, K.
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Journal Title
CELL REPORTS
Volume: 1
Pages: 299-308
DOI
Peer Reviewed
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[Journal Article] Active learning framework with iterative clustering for bioimage classification2012
Author(s)
Kutsuna, N., Higaki, T., Matsunaga, S., Otsuki, T., Yamaguchi, M., Fujii, H. and Hasezawa, S.
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Journal Title
Nature Communications
Volume: 3
Pages: 1032
DOI
Peer Reviewed
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[Presentation] 植物の動原体局在型オーロラキナーゼは微小管プラス末端制御タンパク質EB1をリン酸化する2013
Author(s)
松永幸大, 北原英里奈, 栗原大輔, 小牧伸一郎, 高木麻衣, 佐野行己, 長島慶宜, 中神弘史, 伊藤正樹, 杉本慶子, 橋本隆, 松永朋子, 坂本卓也
Organizer
第36回日本分子生物学会年会
Place of Presentation
神戸ポートアイランド
Year and Date
20131203-06
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[Book] The organisation of genomic DNA in mitotic chromosomes: a novel view. "Molecular Biology and Evolution of the Plant Genome" edited by Greilhuber, J. , Leitch, I. , Wendel, J. and Dolezel, J.2012
Author(s)
Takata, H. , Matsunaga, S. and Maeshima, K.
Total Pages
33-44
Publisher
Springer Verlag (Heidelberg, Germany)
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