Parmales: an insight into the origin and evolutionary success of diatoms

2013 Fiscal Year Final Research Report

• Project/Area Number: 23370046
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biodiversity/Systematics
• Research Institution: Fisheries Research Agency
• Principal Investigator: KUWATA Akira 独立行政法人水産総合研究センター, その他部局等, 主任研究員 (40371794)
• Co-Investigator(Kenkyū-buntansha): SATO Naoki 東京大学, 総合文化研究科, 教授 (40154075) ; SAIYO Kenji 水産総合研究センター, 中央水産研究所, グループ長 (80371798) ; YOSHIKAWA Shinya 福井県立大学, 海洋生物資源学部, 講師 (20405070) ; SAWADA Ken 北海道大学, 理学研究科, 准教授 (20333594)
• Co-Investigator(Renkei-kenkyūsha): ICHINOMIYA Mutsuo 熊本県立大学, 環境共生学部, 講師 (30601918) ; KAWACHI Masanobu 国立環境研究所, 生物生態系研究センター, 室長 (50260469) ; OHKI Kaori 福井県立大学, 海洋生物資源学部, 名誉教授 (90101104) ; NAKAMURA Youji 水産総合研究センター, 中央水産研究所, 研究員 (90463182)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: 藻類 / 進化 / 珪藻 / 海洋生物学 / ゲノム / パルマ藻 / プランクトン

Research Abstract

Parmales are an unknown small marine phytoplankton group with a silicified cell wall. Based on similarities in cell wall structure, Parmales has been proposed as a close relative of diatoms, the most successful microalgal group in modern oceans, and may play a key role in understanding their origin of silica cell wall and early evolution, which have not yet been clearly established. Recently, we isolated the first ever culture of Parmales in the Oyashio region of Japan. Molecular phylogenetic analysis of this culture indicated that Parmales was within the bolidophycean clade of autotrophic naked flagellates and a sister group of diatoms. We have studied on Parmales using ecological, physiological, genomic and biogeochemical approaches and analyzed their phylogenetic relationship between Bolidophycea and diatoms, chloroplast and mitochondria genomes, silica cell wall formation process, population dynamics in the Oyashio region, life cycle and specific biomarkers (molecular fossils).

Research Products

• [Journal Article] Growth characteristics and vertical distribution of Triparma laevis (Parmales) during summer in the Oyashio Region, western North Pacific (2013)
  • Author(s): Mutsuo Ichinomiya, Miwa Nakamachi, Yugo Shimizu, Akira Kuwata
  • Journal Title: Aquatic Microbial Ecology Volume: 68 Pages: 107-116
  • Peer Reviewed
• [Journal Article] Evaluation of paleoenvironment using terpenoid biomarkers in lignites and plant fossils from the Miocene Tokiguchi Porcelain Clay Formation at the Onada mine, Tajimi, central Japan (2013)
  • Author(s): Ken Sawada, Hideto Nakamura, Takaki Arai, Minoru Tsukagoshi
  • Journal Title: International Journal of Coal Geology Volume: 107 Pages: 78-89
• [Journal Article] Statistics of N-terminal alignment as a guide for refining prokaryotic gene annotation (2012)
  • Author(s): Naoki Sato, Naoyuki Tajima
  • Journal Title: Genomics Volume: 99 Pages: 138-143
  • Peer Reviewed
• [Presentation] パルマ藻から珪藻の進化の秘密を探る (2013)
  • Author(s): 桑田晃
  • Organizer: 日本植物学会第77回大会
  • Place of Presentation: 北海道大学, 札幌
  • Year and Date: 2013-09-13
• [Presentation] パルマ藻から見た珪藻の細胞外被形成機構 (2013)
  • Author(s): 山田和正, 神谷充伸, 大城香, 吉川伸哉
  • Organizer: 日本植物学会第77回大会
  • Place of Presentation: 北海道大学, 札幌
  • Year and Date: 2013-09-13
• [Presentation] パルマ藻のゲノム解析 (2013)
  • Author(s): 佐藤直樹, 斉藤憲治
  • Organizer: 日本植物学会第77回大会
  • Place of Presentation: 北海道大学, 札幌
  • Year and Date: 2013-09-13
• [Presentation] パルマ藻の脂質バイオマーカー : 珪藻との関連性 (2013)
  • Author(s): 沢田健, 加納千紗都, 吉川伸哉, 一宮睦雄, 桑田晃
  • Organizer: 日本植物学会第77回大会
  • Place of Presentation: 北海道大学, 札幌
  • Year and Date: 2013-09-13
• [Presentation] 生活史からみたパルマ藻の多様性 (2013)
  • Author(s): 河地正伸, 佐藤真由美, Mary-Helene Noel
  • Organizer: 日本植物学会第77回大会
  • Place of Presentation: 北海道大学, 札幌
  • Year and Date: 2013-09-13
• [Presentation] 親潮および親潮-黒潮混合域におけるパルマ藻の分布と季節変化 (2013)
  • Author(s): 一宮睦雄
  • Organizer: 日本植物学会第77回大会
  • Place of Presentation: 北海道大学, 札幌
  • Year and Date: 2013-09-13
• [Presentation] Sequence and analysis of the chloroplast and mitochondrial complete genomes of the parmales Triparma laevis (2013)
  • Author(s): Tajima N, Saito K, Sato, S, Maruyama F, Ichinomiya M, Yoshikawa Y, Kurokawa K, Ohta H, Tabata S, Kuwata A, Sato N
  • Organizer: The 12th International Colloquiumon Endocytobiology and Symbiosis
  • Place of Presentation: Dalhousie University, Halifax, Canada
  • Year and Date: 2013-08-18
• [Presentation] Exploring the Evolutionary Link Between Parmales and the Success of Diatoms in Marine Ecosystems (2013)
  • Author(s): Kuwata A, Ichinomiya M, Yoshikwa S, Kawachi M, Saitoh K, Sato N, Sawada K
  • Organizer: EMBO Workshop "Molecular life of diatoms"
  • Place of Presentation: Collège de France, Paris, France
  • Year and Date: 2013-06-28