2013 Fiscal Year Final Research Report
Crystal structure of human Band3 (anion exchanger)
| Project/Area Number |
23370049
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
KOBAYASHI TAKUY 京都大学, 医学(系)研究科(研究院), 准教授 (20311730)
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| Co-Investigator(Kenkyū-buntansha) |
HAMASAKI Naotaka 長崎国際大学, 薬学部, 名誉教授 (00091265)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | X線結晶構造解析 / アニオントランスポーター / バンド3 |
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| Research Abstract |
Human AE1 is a 110kDa glycoprotein. It is built from two domains, a cytosolic N-terminal domain (residues 1-360) and an integral membrane domain (residues 361-911). Anion exchange is catalysed by the C-terminal domain. However, only the crystal structure of the cytosolic N-terminal domain, important as an anchoring point for other proteins including the scaffolding protein ankyrin and deoxy-hemoglobin, has been determined. As such the details of the topology, the substrate recognition, and the anion-transport mechanism of this fundamental protein remain unclear. We analyzed the crystal structure at 3.4 angstrom resolution of the membrane domain of human AE1 locked in an outward open conformation by a covalently bound H2DIDS-inhibitor in complex with a Fab fragment from a monoclonal antibody. We also need the structure of an inward-facing open conformation or an outward-facing open conformation at higher resolution to understand the fully anion exchange mechanism of AE1.
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Author(s)
Murai, Y., Masuda, K., Ogasawara, Y., Wang, L., Hashidoko, Y., Hatanaka, Y., Iwata, S., Kobayashi, T., and Hashimoto, M
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Journal Title
Eur. J. Org. Chem
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Pages: 2428-2433
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Haga, K., Kruse, A.C., Asada, H., Yurugi-Kobayashi, T., Shiroishi, M., Zhang, C., Weis, W.I., Okada, T., Kobilka, B.K., Haga, T. and Kobayashi, T
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Journal Title
Structure of the human M2 muscarinic acetylcholine receptor bound to an antagonist
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[Journal Article] Platform for the rapid construction and evaluation of GPCRs for crystallography in Saccharomyces cerevisiae2012
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Shiroishi, M., Tsujimoto, H., Makyio, H., Asada, H., Yurugi-Kobayashi, T., Shimamura, T., Nomura, N., Haga, T., Iwata, S. and *Kobayashi, T
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Microb. Cell Fact
Volume: 11
Pages: 78
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[Journal Article] Production of the stable human histamine H1 receptor in Pichia pastoris for structural determination2012
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Shiroishi, M., Kobayashi, T., Ogasawara, S., Tsujimoto, H., Ikeda-Suno, C., Iwata, S. and Shimamura, T
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Volume: 55
Pages: 281-286
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[Journal Article] protein-coupled receptor inactivation by an allosteric inverse-agonist antibody2012
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Hino, T., Arakawa, T., Iwanari, H., Yurugi-Kobayashi, T., Ikeda-Suno, C., Nakada-Nakura, Y., Kusano-Arai, O., Weyand, S., Shimamura, T., Nomura, N., Cameron, A.D., Kobayashi, T., Hamakubo, T., Iwata, S. and Murata, T. G
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Journal Title
Nature
Volume: 482
Pages: 237-240
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[Journal Article] Structure of the human histamine H1 receptor complex with doxepin2011
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Shimamura, T., Shiroishi, M., Weyand, S., Tsujimoto, H., Winter, G., Katritch, V., Abagyan, R., Cherezov, V., Liu, W., Han, G.W., Kobayashi, T., Stevens, R.C. and Iwata, S
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Journal Title
Nature
Volume: 475
Pages: 65-70
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[Journal Article] Cloning, expression and purification of the anion exchanger 1 homologue from basidiomycete Phanerochaete chrysosporium2011
Author(s)
Tokuda, N., Igarashi, K., Shimamura, T., Yurugi-Kobayashi, T., Shiroishi, M., Ito, K., Sugawara, T., Asada, H., Murata, T., Nomura, N., Iwata, S. and Kobayashi, T
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Journal Title
Protein Expr. Purif
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[Journal Article] Evaluation of the Pichia pastoris expression system for the production of GPCRs for structural analysis2011
Author(s)
Asada, H., Uemura, T., Yurugi-Kobayashi, T., Shiroishi, M., Shimamura, T., Tsujimoto, H., Ito, K., Sugawara, T., Nakane, T., Nomura, N., Murata, T., Haga, T., Iwata, S. and Kobayashi, T
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Journal Title
Microb. Cell Fact
Volume: 10
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[Journal Article] Cysteine-to-serine shuffling using a yeast expression system improves protein secretion : case of a nonglycosylated mutant of miracullin, a taste-modifying protein2011
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Ito, K., Sugawara, T., Koizumi, A., Nakajima, K., Shimizu-Ibuka, A., Shiroishi, M., Asada, H., Yurugi-Kobayashi, T., Shimamura, T., Asada, T., Misaka, T., Iwata, S., Kobayashi, T. and Abe, K
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Journal Title
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Volume: 33
Pages: 103-107
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[Journal Article] Crystal structure of Glucansucrase from the dental caries pathogen Streptococcus mutans2011
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Ito, K., Ito, S., Shimamura, T., Weyand, S., Kawarasaki, Y., Misaka, T., Abe, K., Kobayashi, T., Cameron, A.D., and Iwata S
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Journal Title
J. Molecular Biology
Volume: 408
Pages: 177-186
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[Patent(Industrial Property Rights)] 抗ヒトバンド3モノクローナル抗体2011
Inventor(s)
小林拓也, 小林貴美, 荒川孝俊, 日野智也, 村田武士, 野村紀通, 岩田想, 浜窪隆雄, 岩成宏子, 望月康弘, 濱崎直孝
Industrial Property Rights Holder
独立行政法人科学技術振興機構
Industrial Property Rights Type
特願
Industrial Property Number
2011-246364
Filing Date
2011-11-10
