2013 Fiscal Year Final Research Report
Carbohydrate recognition mechanisms of galectins and carbohydrate processing enzymes deduced from X-ray structures
| Project/Area Number |
23370054
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Kagawa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Hiromi 香川大学, 総合生命科学研究センター, 准教授 (10313305)
NISHI Nozumu 香川大学, 総合生命科学研究センター, 准教授 (10145047)
NAKAKITA Shinichi 香川大学, 総合生命科学研究センター, 准教授 (40314356)
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| Co-Investigator(Renkei-kenkyūsha) |
MIYATA Shigeru 中部大学, 応用生物学部, 准教授 (90314913)
HOJO Hironobu 大阪大学, 蛋白質研究所, 教授 (00209214)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | X線結晶解析 / 糖鎖 / 糖鎖結合タンパク質 / ガレクチン / ヘマグルチニン / エンドライシン / ウェルシュ菌 / ボツリヌス菌 |
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| Research Abstract |
Cell surface oligosaccharides are involved in immune responses and bacterial/viral infections, and they are specifically recognized by carbohydrate-binding proteins. X-ray structures of the complexes of carbohydrate-binding proteins with the target oligosaccharides have been determined to elucidate the carbohydrate recognition mechanisms. The results of this research project give new insights into the carbohydrate recognition mechanisms of galectin-8, a hemagglutinin subcomponent of the botulinum neurotoxin, and an endolysin encoded by episomal phage of Clostridium perfringens.
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[Journal Article] X-ray structure of a novel endolysin encoded by episomal phage phiSM101 of Clostridium perfringens2014
Author(s)
Tamai, E., Yoshida, H., Sekiya, H., Nariya, H., Miyata, S., Okabe, A., Kuwahara, T., Maki, J. & Kamitori, S
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Journal Title
Mol. Microbiol
Volume: 92
Pages: 326-337
DOI
Peer Reviewed
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[Journal Article] Self-association of the galectin-9 C-terminal domain via the opposite surface of the sugar-binding site2013
Author(s)
Nonaka, Y., Ogawa, T., Oomizu, S., Nakakita, S., Nishi, N., Kamitori, S., Hirashima, M. & Nakamura, T
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Journal Title
J. Biochem
Volume: 153
Pages: 463-471
DOI
Peer Reviewed
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[Journal Article] X-ray structure of a protease-resistant mutant form of human galectin-8 with two carbohydrate recognition domains2012
Author(s)
Yoshida, H., Yamashita, S., Teraoka, M., Itoh, A., Nakakita, S., Nishi, N. & Kamitori, S.
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Journal Title
FEBS J
Volume: 279
Pages: 3937-3951
DOI
Peer Reviewed
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[Journal Article] Carbohydrate recognition mechanism of HA70 from Clostridium botulinum deduced from X-ray structures in complexes with sialylated oligosaccharides2012
Author(s)
Yamashita, S., Yoshida, H., Uchiyama, N., Nakakita, Y., Nakakita, S., Tonozuka, T., Oguma, K., Nishikawa, A. & Kamitori, S
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Journal Title
FEBS Lett
Volume: 586
Pages: 2404-2410
DOI
Peer Reviewed
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