2013 Fiscal Year Final Research Report
TECHNOLOGY OF NMR SIGNAL ASSIGNMENTS OF PROTEINS WITH HIGH SENSITIVITY
| Project/Area Number |
23370056
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 生体高分子 / NMR / タンパク質 / 安定同位体 / 微量解析 / 無細胞タンパク質合成 |
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| Research Abstract |
We have improved and/or developed two methods for NMR signal assignments of large proteins with less amount of sample. At first, we improved NMR pulse programs for MAGICAL method that we previously developed with the intelligent stable isotope labeling techniques. As a result, the sensitivity of NMR measurements was dramatically enhanced by 30%. Second, we developed a new method for site-directed stable isotope labeling by changing two kinds of tRNAs of major codon and minor codon for the same amino acid. With these methods, it was indicated that NMR signals of proteins could be assigned which were difficult to analyze by using traditional NMR techniques.
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