2013 Fiscal Year Final Research Report
Study of pH homeostasis in acidic organelles
| Project/Area Number |
23370060
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Osaka University |
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Principal Investigator |
MAEDA YUSUKE 大阪大学, 微生物病研究所, 准教授 (00294124)
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| Co-Investigator(Renkei-kenkyūsha) |
KANZAWA Noriyuki 大阪大学, 微生物病研究所, 特任助教 (40452461)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | ゴルジ装置 / pH / コレステロール / 糖鎖修飾 / 輸送 / 膜コンタクト部位 / GPHR |
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| Research Abstract |
By analyzing GPHR-deficient mouse embryonic fibroblast (MEF) in which major pathology is a raised Golgi pH, we clarified 1) impaired glycosylation is caused by reduced activities of nucleotide-sugar transporter and aberrant complex formation of glycosyltransferases but not by mislocalization of glycosyltransferases, 2) both transcriptions of several genes involved in cholesterol biosynthesis and the amount of intracellular cholesterol are reduced in GPHR-deficient MEF and it is most likely that the phenotype is caused by abnormal cholesterol distribution due to the impaired cholesterol trafficking through the membrane contact site between the ER and Golgi apparatus, and 3) skin basal cell-specific gene targeting revealed that GPHR is essential for the homeostasis of the epidermis including the formation of lamellar bodies and for the barrier function.
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