2014 Fiscal Year Final Research Report
Structure modeling and functional analysis of rotary motor state proteins based on mutational information
| Project/Area Number |
23370066
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KITAO Akio 東京大学, 分子細胞生物学研究所, 准教授 (30252422)
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| Project Period (FY) |
2011-04-01 – 2015-03-31
|
| Keywords | 生体膜 / 受容体 / チャネル / 立体構造モデリング |
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| Outline of Final Research Achievements |
We have conducted a structure modeling of the transmembrane part of the stator complex MotA/B in the flagellar motor of Escherichia coli and investigated the proton permeation process. We found that hydrophobic channels are formed in MotA/B and protons are transported as hydronium ions through the channel. We also found that protonation/deprotonation of Asp32 can induce a ratchet motion of the cytoplasmic domain. This research result is now in press in the Proc Natl Acad Sci USA.
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| Free Research Field |
生物物理学、計算科学、情報生物学
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