2013 Fiscal Year Final Research Report
Experimental analysis of conformational evolution process of a prion by developing the kinetic NMR
| Project/Area Number |
23370068
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Gifu University |
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Principal Investigator |
KUWATA Kazuo 岐阜大学, 連合創薬医療情報研究科, 教授 (00170142)
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| Co-Investigator(Kenkyū-buntansha) |
KAMATARI Yuji 岐阜大学, 生命科学総合研究支援センター, 助教 (70342772)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | NMR / キネティクス / プリオン / 立体構造進化 / 活性化 |
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| Research Abstract |
In order to elucidate the conformational conversion process of a prion protein from its cellular form to the pathogenic one and the prion's evolution mechanism, we created a series of novel apparatuses, i.e. kinetic fluorescence, kinetic CD, kinetic DLS, and kinetic NMR (800 MHz). Using these novel analytical systems, we could detect the early conformational changes in a prion protein prior to the aggregation formation. Using the kinetic DLS, we detected the transient formation of disordered assembly before the stable amyloid-like aggregate formation. Using the kinetic NMR, we could detect the region specific degradation of the peptide bonds by imposing the ultrasonication. Those phenomena would give us important clues for understanding the mechanism of conformational conversion as well as that of evolution.
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Volume: 21(21)
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Journal Title
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Volume: 2012
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[Journal Article] Deciphering the Molecular Details for the Binding of the Prion Protein to Main Ganglioside GM1 of Neuronal Membranes2011
Author(s)
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Journal Title
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Pages: 1422-1431
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Peer Reviewed
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