2013 Fiscal Year Final Research Report
Activation mechanisms of G-protein-coupled receptors based on the structural dynamics
| Project/Area Number |
23370070
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Biophysics
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
IMAMOTO Yasushi 京都大学, 理学(系)研究科(研究院), 准教授 (80263200)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SHICHIDA Yoshinori 京都大学, 大学院・理学研究科, 教授 (60127090)
YAMASHITA Takahiro 京都大学, 大学院・理学研究科, 助教 (50378535)
SAKO Yasushi 理化学研究所, 主任研究員 (20215700)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
OKA Toshihiko 静岡大学, 大学院・理学研究科, 講師 (60344389)
|
|---|
| Project Period (FY) |
2011-04-01 – 2014-03-31
|
| Keywords | 平衡 / 一分子計測 / X線高角散乱 / 赤外分光 / 構造変化 / アゴニスト / 構成的活性変異 |
|---|
| Research Abstract |
The activation process of G protein-coupled receptors was investigated based on the structural dynamics using rhodopsin. The effect of the ligand on the structure and equilibrium of the active state was analyzed by high-angle solution X-ray scattering, single-molecule fluorometry, and Fourier-transform infrared spectroscopy. Our results demonstrated that G protein-coupled receptors are in the equilibrium between active and inactive conformations, and the inverse-agonist and agonist bias the equilibrium toward inactive and active conformations, respectively, to regulate the activity.
|
Research Products
(13 results)
-
[Journal Article] Mapping of the local environmental changes in proteins by cysteine scanning2014
Author(s)
Yamazaki, Y., Nagata, T., Terakita, A., Kandori, H., Shichida, Y., and Imamoto, Y
-
Journal Title
Biophysics
Volume: 10
Pages: 1-7
DOI
Peer Reviewed
-
[Journal Article] Ligand-induced population shift leads to the activation of rhodopsin, a G-protein coupled receptor2014
Author(s)
Maeda, R., Hiroshima, M., Yamashita, T., Wada, A., Nishimura, S., Sako, Y., Shichida, Y., and Imamoto, Y
-
Journal Title
Biophys. J
Volume: 106 (4)
Pages: 915-924
DOI
Peer Reviewed
-
-
[Journal Article] Intramolecular interactions that induce helical rearrangement upon rhodopsin activation : Light-induced structural changes in metarhodopsin Iia probed by cysteine S-H stretching vibrations2014
Author(s)
Yamazaki, Y., Nagata, T., Terakita, A., Kandori, H., Shichida, Y., and Imamoto, Y.
-
Journal Title
J. Biol. Chem
Volume: 289 (20)
Pages: 13792-13800
DOI
Peer Reviewed
-
-
-
-
-
[Presentation] Characterization of visual pigments in membrane environment using nanodiscs2013
Author(s)
Imamoto, Y., Kojima, K., Maeda, R., Oka, T., Yamashita, T., and Shichida, Y
Organizer
The 6th Asia & Oceania Conference on Photobiology
Place of Presentation
Novotel Sydney Central, Sydney, Australia
Year and Date
2013-11-11
-
[Presentation] Lightinduced structural changes of rhodopsin probed by cysteine S-H stretching vibrations2012
Author(s)
Imamoto, Y., Yamazaki, Y., Nagata, T., Terakita, A., Kandori, H., and Shichida, Y
Organizer
15th International Conference on Retinal Proteins
Place of Presentation
Monte Verità, Ascona, Switzerland
Year and Date
2012-10-01
-
-
-
