Integrated understanding of regulations of DNA replication by cell cycle and chromatin

2013 Fiscal Year Final Research Report

• Project/Area Number: 23370076
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Osaka University
• Principal Investigator: MASUKATA Hisao 大阪大学, 理学(系)研究科(研究院), 教授 (00199689)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: DNA複製 / 細胞周期 / クロマチン / テロメア / 分裂酵母

Research Abstract

We investigated the molecular mechanisms how initiation of DNA replication is regulated by cell cycle and chromatin structures using model organism fission yeast. We found that phosphorylations of multiple replication factors, Sld3 and Drc1, by cell cycle master kinase CDK are crucial to trigger assembly of replication factors at replication origins. We further discovered the role of Mcm10 after assembly of replication factors to initiate actual DNA synthesis. In approach to elucidate the replication timing program, we discovered that telomere-repeat sequence is located near the late-replicating origins and the telomere-binding proteins are involved in replication timing program. These findings are published on high-quality international journals.

Research Products

• [Journal Article] Fission yeast RecQ helicase Rqh1 is required for the maintenance of circular chromosomes (2013)
  • Author(s): Nanbu, T., Takahashi, K., Murray, IM., Hirata, N., Ukimori, S., Kanke, M., Masukata, H., Yukawa, M., Tsuchiya, E., Ueno, M
  • Journal Title: Mol. Cell. Biol Volume: 33(6) Pages: 1175-1187
  • DOI: 10.1128/MCB.01713-12.
  • Peer Reviewed
• [Journal Article] Telomere-binding proteins play roles in control of replication timing (2012)
  • Author(s): Tazumi, A., Masukata, H.
  • Journal Title: Cell Cycle Volume: 11(24) Pages: 4492-4493
  • DOI: 10.1101/gad.194282.112
  • Peer Reviewed
• [Journal Article] Telomere-binding protein Taz1 controls global replication timing through its localization near late replication origins in fission yeast (2012)
  • Author(s): Tazumi, A., Fukuura, M., Nakato, R., Kishimoto, A., Takenaka, T., Ogawa, S., Song, J., Takahashi, TS., Nakagawa, T., Masukata, H
  • Journal Title: Genes Dev Volume: 26(18) Pages: 2050-2062
  • DOI: 10.1101/gad.194282.112
  • Peer Reviewed
• [Journal Article] DNA polymerization-independent functions of DNA polymerase epsilon in assembly and progression of the replisome in fission yeast (2012)
  • Author(s): Handa, T., Kanke, M., Takahashi, TS, Nakagawa, T., Masukata, H
  • Journal Title: Mol. Biol. Cell Volume: 23(16) Pages: 3240-3253
  • DOI: 10.1091/mbc.E12-05-033
  • Peer Reviewed
• [Journal Article] The prereplication complex recruits XEco2 to chromatin to promote cohesin acetylation in Xenopus egg extracts (2012)
  • Author(s): Higashi, TL., Ikeda, M., Tanaka, H., Nakagawa, T., Bando, M., Shirahige, K., Kubota, Y., Takisawa, Masukata, H., Takahashi, TS
  • Journal Title: Curr Biol Volume: 22 (11) Pages: 977-988
  • DOI: 10.1016/j.cub.2012.04.013
  • Peer Reviewed
• [Journal Article] Mcm10 plays an essential role in origin unwinding after loading of the CMG components (2012)
  • Author(s): Kanke, M., Kodama, Y., Takahashi, TS., Nakagawa, T., Masukata, H
  • Journal Title: EMBO J Volume: 31 Pages: 2182-2194
  • DOI: 10.1038/emboj.2012.68
  • Peer Reviewed
• [Journal Article] An abundance of pre-replicative complexes (pre-RCs) facilitates recombinational repair under replication stress in fission yeast (2011)
  • Author(s): Maki, K., Inoue, T., Onaka, A., Hashizume, H., Somete, N., Kobayashi, Y., Murakami, S., Shigaki, C., Takahashi, TS., Masukata, H., Nakagawa, T
  • Journal Title: J. Biol. Chem. Volume: 286(48) Pages: 41701-41710
  • DOI: 10.1074/jbc.M111.285619
  • Peer Reviewed
• [Journal Article] CDK promotes interactions of Sld3 and Drc1 for initiation of DNA replication in fission yeast (2011)
  • Author(s): Fukuura, M., Nagao, K., Obuse, C., Takahashi, TS., Nakagawa, T., Masukata, H
  • Journal Title: Mol. Biol. Cell Volume: 22 Pages: 2620-2633
  • Peer Reviewed
• [Journal Article] Auxin-inducible protein depletion system in fission yeast (2011)
  • Author(s): Kanke, M., Nishimura, K., Kanemaki, M., Kakimoto, T., Takahashi, TS., Nakagawa, T., Masukata, H
  • Journal Title: BMC Cell Biol Volume: 12: 8
  • DOI: 10.1186/1471-2121-12-8
  • Peer Reviewed
• [Journal Article] Regulation of DNA replication by chromatin structures : accessibility and recruitment (2011)
  • Author(s): Hayashi, MT., Masukata, H
  • Journal Title: Chromosoma Volume: 120 Pages: 39-46
  • Peer Reviewed
• [Presentation] Regulation of replication program in fission yeast : replicator, initiator and regulators (2013)
  • Author(s): 升方久夫
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 20131203-06
• [Presentation] Replication timing control by chromatin proteins (2013)
  • Author(s): 升方久夫
  • Organizer: Eukaryotic DNA replication and genome maintenance
  • Place of Presentation: Cold Spring Harbor, NY, USA
  • Year and Date: 20130909-13
• [Presentation] Replication timing control by centromere and telomere binding proteins (2013)
  • Author(s): 升方久夫
  • Organizer: 酵母エピジェネティックミーティング
  • Place of Presentation: 福井県あわら市
  • Year and Date: 20130902-04
• [Presentation] Replication timing program in fission yeast (2013)
  • Author(s): 升方久夫
  • Organizer: 第7回分裂酵母国際学会
  • Place of Presentation: London UK
  • Year and Date: 20130624-29
• [Presentation] Replication timing control by telomere proteins (2013)
  • Author(s): 升方久夫
  • Organizer: 第21回国際遺伝学会・Human Genome Meeting 2013
  • Place of Presentation: Singapore
  • Year and Date: 20130412-15
• [Presentation] Replication Timing Program in Fission Yeast (2012)
  • Author(s): 升方久夫
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 20121211-14
• [Presentation] Control of global replication timing by telomere-binding proteins in fission yeast (2012)
  • Author(s): 升方久夫
  • Organizer: 第8回3Rシンポジウム
  • Place of Presentation: 淡路夢舞台
  • Year and Date: 20121125-28
• [Presentation] Activation of replication origins and involvement of telomere proteins in timing control (2012)
  • Author(s): 升方久夫
  • Organizer: DNA replication of eukaryotic genomes at the Foundation des Treilles
  • Place of Presentation: Les Treilles, Les Arcs, France
  • Year and Date: 20120702-07
• [Presentation] Telomere-binding protein Taz1 controls replication timing of the internal late origins in fission yeast (2011)
  • Author(s): 升方久夫
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-15
• [Presentation] Replication timing control by Taz1 that binds to telomeric repeats proximal to internal late replication origins (2011)
  • Author(s): 升方久夫
  • Organizer: Eukaryotic DNA Replication and Genome Maintenance
  • Place of Presentation: Cold Spring Harbor, NY, USA
  • Year and Date: 2011-09-07
• [Presentation] Regulation of replication of fission yeast genome (2011)
  • Author(s): 升方久夫
  • Organizer: The Sixth International Fission Yeast Meeting
  • Place of Presentation: Boston, USA
  • Year and Date: 2011-06-27
• [Remarks]
  • URL: http://www.bio.sci.osaka-u.ac.jp/dbs01/re-paper-temp.php?id=14
• [Remarks]
  • URL: http://www.bio.sci.osaka-u.ac.jp/bio_web/lab_page/masukata/thesis/index.html