2014 Fiscal Year Final Research Report
Dynamics of human genome DNA in living cells revealed by nucleosome imaging
| Project/Area Number |
23370078
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
MAESHIMA Kazuhiro 国立遺伝学研究所, 構造遺伝学研究センター, 教授 (00392118)
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| Project Period (FY) |
2011-04-01 – 2015-03-31
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| Keywords | ゲノム / 遺伝学 / 核酸 / 生物物理 |
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| Outline of Final Research Achievements |
Genome information, which is three-dimensionally organized within cells as chromatin, is searched and read out by various proteins for diverse cell functions. Although how the protein factors find their targets remains unclear, the dynamic nature of chromatin is likely crucial. To pursue this dynamics, we investigated the movement of each nucleosome in the chromatin. It had been technically challenging to image single nucleosome, because a single cell has numerous nucleosomes (≈3 x 107). To fluorescently label a small number of nucleosomes, we fused PA-GFP (PhotoActivatable-GFP) to histone H4, and stably expressed the PA-GFP-H4 in the cells. From our analysis of the single nucleosome movement, we found a local fluctuation of individual nucleosomes. This local dynamic movement would be a basis of various genome events.
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| Free Research Field |
細胞生物学 生物物理学
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