2013 Fiscal Year Final Research Report
Slow axonal transport driven by directional actin turnover
| Project/Area Number |
23370088
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Cell biology
|
|---|
| Research Institution | Nara Institute of Science and Technology |
|---|
Principal Investigator |
INAGAKI Naoyuki 奈良先端科学技術大学院大学, バイオサイエンス研究科, 准教授 (20223216)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
SUGIURA Tadao 奈良先端科学技術大学院大学, 情報科学研究科, 准教授 (60304010)
SAKUMURA Yuichi 愛知県立大学, 情報科学部, 准教授 (50324968)
TORIYAMA Michinori 奈良先端科学技術大学院大学, バイオサイエンス研究科, 研究員 (90457151)
|
|---|
| Project Period (FY) |
2011-04-01 – 2014-03-31
|
| Keywords | 脳・神経 / 発生・分化 / 神経科学 / 軸索輸送 / 細胞骨格 / 分子クラッチ / Shootin1 / アクチン線維 |
|---|
| Research Abstract |
Although actin and associated proteins are essential for axonal extension, how they are transported along axons remains unclear. Here we show that actin filaments (F-actins) and associated proteins migrate toward the axonal growth cone by means of directional actin polymerization/depolymerization, called treadmilling. F-actins migrating along axonal shafts underwent treadmilling, with their polymerizing ends oriented toward the growth cone. F-actins were anchored to the plasma membrane through the cell adhesion molecule L1-CAM and the linker protein shootin1. Migration of F-actins depended on their polymerization and substrate anchorage, and directional counter-forces associated with F-actin migration were detected on the substrate. Actin-associated proteins co-migrated with F-actins by interacting with the filaments. Our findings reveal a new mode of intracellular transport, which supplies actin and associated proteins to the axonal leading edge, thereby promoting its protrusion.
|
-
[Journal Article] Ceramic coating of liposomal gene carrier for minimizing toxicity to primary hippocampal neurons2013
Author(s)
Tahara, K., Moriuchi, T., Tsukui, M., Hirota, A., Maeno, T., Toriyama, M., Inagaki, N. and Kikuchi, J
-
Journal Title
Chemistry Letters
Volume: 42
Pages: 1265-1267
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
