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2013 Fiscal Year Final Research Report

Slow axonal transport driven by directional actin turnover

Research Project

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Project/Area Number 23370088
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionNara Institute of Science and Technology

Principal Investigator

INAGAKI Naoyuki  奈良先端科学技術大学院大学, バイオサイエンス研究科, 准教授 (20223216)

Co-Investigator(Renkei-kenkyūsha) SUGIURA Tadao  奈良先端科学技術大学院大学, 情報科学研究科, 准教授 (60304010)
SAKUMURA Yuichi  愛知県立大学, 情報科学部, 准教授 (50324968)
TORIYAMA Michinori  奈良先端科学技術大学院大学, バイオサイエンス研究科, 研究員 (90457151)
Project Period (FY) 2011-04-01 – 2014-03-31
Keywords脳・神経 / 発生・分化 / 神経科学 / 軸索輸送 / 細胞骨格 / 分子クラッチ / Shootin1 / アクチン線維
Research Abstract

Although actin and associated proteins are essential for axonal extension, how they are transported along axons remains unclear. Here we show that actin filaments (F-actins) and associated proteins migrate toward the axonal growth cone by means of directional actin polymerization/depolymerization, called treadmilling. F-actins migrating along axonal shafts underwent treadmilling, with their polymerizing ends oriented toward the growth cone. F-actins were anchored to the plasma membrane through the cell adhesion molecule L1-CAM and the linker protein shootin1. Migration of F-actins depended on their polymerization and substrate anchorage, and directional counter-forces associated with F-actin migration were detected on the substrate. Actin-associated proteins co-migrated with F-actins by interacting with the filaments. Our findings reveal a new mode of intracellular transport, which supplies actin and associated proteins to the axonal leading edge, thereby promoting its protrusion.

Research Products

(19 results)

All 2014 2013 2012 2011 Other

All Journal Article Presentation Book Remarks

  • [Journal Article] Ceramic coating of liposomal gene carrier for minimizing toxicity to primary hippocampal neurons2013

    • Author(s)
      Tahara, K., Moriuchi, T., Tsukui, M., Hirota, A., Maeno, T., Toriyama, M., Inagaki, N. and Kikuchi, J
    • Journal Title

      Chemistry Letters

      Volume: 42 Pages: 1265-1267

    • Peer Reviewed
  • [Journal Article] プロテオミクスを基礎にした神経細胞が非対称性を獲得する機構の解析2012

    • Author(s)
      吉田亙, 鳥山道則, 稲垣直之
    • Journal Title

      生物物理化学

      Volume: 56 Pages: 31,1-4

    • Peer Reviewed
  • [Presentation] Axonal transport toward growth cones driven by directional actin turnover2014

    • Author(s)
      勝野弘子, 鳥山道則, 作村諭一,池田和司,水野健作,稲垣直之
    • Organizer
      生命分子システムにおける動的秩序形成と高次機能発現第2回公開シンポジウム
    • Place of Presentation
      京都市
    • Year and Date
      20140111-12
  • [Presentation] アクチンのトレッドミルによって引き起こされる遅い軸索輸送2013

    • Author(s)
      勝野弘子, 鳥山道則, 作村諭一, 池田和司, 水野健作, 稲垣直之
    • Organizer
      Neuro2013
    • Place of Presentation
      京都市
    • Year and Date
      20130620-23
  • [Presentation] 脳発生における Shootin1 および Shootin2 の機能解析2013

    • Author(s)
      吉田亙, 島田忠之, 鳥山道則, 河野憲二, 稲垣直之
    • Organizer
      第65回日本細胞生物学会大会
    • Place of Presentation
      愛知県名古屋市
    • Year and Date
      20130619-21
  • [Presentation] 分子輸送の不規則性が神経形態を極性化する2013

    • Author(s)
      作村諭一, 前野貴則, 稲垣直之
    • Organizer
      第65回日本細胞生物学会大会
    • Place of Presentation
      愛知県名古屋市
    • Year and Date
      20130619-21
  • [Presentation] 軸索伸長のためのシグナル-力変換機構2013

    • Author(s)
      稲垣直之
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      京都市
    • Year and Date
      2013-10-29
  • [Presentation] Cortactin functions as a clutch molecule to promote axon outgrowth2013

    • Author(s)
      久保祐亮, 鳥山道則, 小沢哲, 池田和司, 杉浦忠男, 稲垣直之
    • Organizer
      International Symposium on "Sensory Systems and Neural Circuits"
    • Place of Presentation
      東京大学
    • Year and Date
      2013-02-11
  • [Presentation] Slow axonal transport driven by directional actin treadmilling2012

    • Author(s)
      勝野弘子, 鳥山道則, 作村諭一, 池田和司, 水野健作, 稲垣直之
    • Organizer
      2012 Annual Meeting of the American Society for Cell Biology
    • Place of Presentation
      San Francisco, USA
    • Year and Date
      2012-12-18
  • [Presentation] Cortactin functions as a clutch molecule to promote axon outgrowth2012

    • Author(s)
      久保祐亮, 鳥山道則, 小沢哲, 池田和司, 杉浦忠男, 稲垣直之
    • Organizer
      2012 Annual Meeting of the American Society for Cell Biology
    • Place of Presentation
      San Francisco, USA
    • Year and Date
      2012-12-16
  • [Presentation] Slow axonal transport driven by directional actin treadmilling of anchored actin filaments2012

    • Author(s)
      勝野弘子, 鳥山道則, 作村諭一, 池田和司, 水野健作, 稲垣直之
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      愛知県名古屋市
    • Year and Date
      2012-09-19
  • [Presentation] Mecano-systems biological analysis of neuronal polarity formation2012

    • Author(s)
      稲垣直之
    • Organizer
      ドイツ生化学分子生物学会( GBM ) Perspectives in Morecular Neurobiology
    • Place of Presentation
      Bochum, Germany
    • Year and Date
      2012-09-14
  • [Presentation] Cortactin functions as a clutch molecule to promote axon outgrowth2012

    • Author(s)
      久保祐亮, 鳥山道則, 小沢哲, 池田和司, 杉浦忠男, 稲垣直之
    • Organizer
      第45回日本発生生物学会・第64回日本細胞生物学会合同大会
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2012-05-29
  • [Presentation] Mechanisms for the axon- specific accumulation of an axonal protein JIP12011

    • Author(s)
      前野貴則, 鳥山道則, 稲垣直之
    • Organizer
      第34回日本分子生物学会
    • Place of Presentation
      横浜市
    • Year and Date
      2011-12-16
  • [Presentation] Cortactin functions as a clutch molecule to promote axon outgrowth2011

    • Author(s)
      久保祐亮, 鳥山道則, 稲垣直之
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜市
    • Year and Date
      2011-12-16
  • [Presentation] Analysis of the mechanism of axonal transport slow component2011

    • Author(s)
      勝野弘子, 鳥山道則, 稲垣直之
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜市
    • Year and Date
      2011-12-15
  • [Presentation] プロテオミクスを基礎にした神経細胞が非対称性を獲得する機構の解析2011

    • Author(s)
      稲垣直之
    • Organizer
      第62回日本電気泳動学会シンポジウム「電気泳動を基礎にした臨床プロテオミクス最近の話題」
    • Place of Presentation
      横浜市
    • Year and Date
      2011-11-13
  • [Book] 脳科学辞典(田中啓治,御子柴克彦編)2012

    • Author(s)
      久保祐亮, 稲垣直之
    • Publisher
      CRMP2
    • URL

      http://bsd.neuroinf.jp/wiki/CRMP

  • [Remarks]

    • URL

      http://nippon.naist.jp/inagaki_g/

URL: 

Published: 2015-06-25  

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