2013 Fiscal Year Final Research Report
Analysis of the proteins possessed by the last common ancestor and the organisms present before it
| Project/Area Number |
23370097
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Evolutionary biology
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| Research Institution | Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YOKOBORI Shin-ichi 東京薬科大学, 生命科学部, 講師 (40291702)
AKANUMA Satoshi 東京薬科大学, 生命科学部, 助教 (10321720)
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| Project Period (FY) |
2011-11-18 – 2014-03-31
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| Keywords | 生命初期進化 / 共通祖先 / コモノート / NDK / 分子系統樹 / タンパク質耐熱性 / 酵素活性 |
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| Research Abstract |
We have resurrected the gene of the nucleotidediphosphate kinase (NDK) possessed by the common ancestor of Bacteria and Archaea, and analyzed the thermal stability of the protein produced from the resurrected genes. The ancestral NDKs showed stability close to 110 degree C, suggesting the common ancestors of Bacteria and Archaea were thriving at the temperature between 80 and 100 degree C. The NDK of the last common ancestor, commonote, was also resurrected and reproduced. The ancestral NDK showed the high stability, suggesting that the commonote were living at 75 degree C or higher temperature. The ancestral NDKs from which one of the 20 amino acid species were removed, were produced and whose stability and activity were analyzed. The results showed the amino acid species that is not absolutely required for the stability and activity.
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[Journal Article] Experimental evidence for the thermophilicity of ancestral life2013
Author(s)
Akanuma, S., Nakajima, Y., Yokobori, S., Kimura, M., Nemoto, N., Mase, T., Miyazono, K., Tanokura, M., Yamagishi A
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Journal Title
Proc. Natl. Acad. Sci. USA
Volume: 110(27)
Pages: 11067-11072
Peer Reviewed
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[Journal Article] Phylogeny-based design of a B-subunit of DNA gyrase and its ATPase domain using a small set of homologous amino acid sequences2011
Author(s)
Akanuma, S., Iwami, S., Yokoi, T., Nakamura, N., Watanabe, H., Yokobori, S. and Yamagishi, A
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Journal Title
J. Mol. Biol
Volume: 412,(2)
Pages: 212-225
Peer Reviewed
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[Remarks] 山岸明彦,全生物の共通祖先となる高温安定タンパク質の復元, 科研費 NEWS 3: 15(2013)
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[Remarks] 玉腰雅忠,八木創太,赤沼哲史,山岸明彦,高機能タンパク質創製技術の現状と展望, 「技術予測レポート 2023」 上巻,日本能率協会総合研究所 80-91(2013)
