Study on a network of genes related to flowering and growth habit and its responses to environments in soybean

2013 Fiscal Year Final Research Report

• Project/Area Number: 23380001
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Breeding science
• Research Institution: Hokkaido University
• Principal Investigator: ABE Jun 北海道大学, (連合)農学研究科(研究院), 教授 (00192998)
• Co-Investigator(Kenkyū-buntansha): YAMADA Testuya 北海道大学, 大学院・農学研究院, 講師 (70374618)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: ダイズ / 開花 / 感光性 / 遺伝子発現 / 遺伝的多様性 / 熟期遺伝子 / FLOWERING LOCUS T

Research Abstract

Soybean is cultivated across a wide range of latitudes. Its wide adaptability is supported by a genetic diversity in the control of flowering. The purposes of this project were to reveal the genetic diversity at four flowering loci, E1 to E4 and to determine the genetic network of flowering genes and the responses to environments. There were three mechanisms in the control of photoperiod-insensitivity; dysfunction of phytochrome A genes, E3 and E4, dysfunction of E1, a possible transcriptional factor for FLOWERING LOCUS T (FT) orthologs, and an involvement of a novel gene(s). Two homologs of E1 inhibited flowering via down-regulation of FT, as in E1. Further, a light signal in a specific period of day induced the gene expression of E1 under the control of E3 and E4 to inhibit the induction of FT expressions, which consequently inhibited the floral induction. A pathway independent of FT may also be involved in the induction of flowering in soybean.

Research Products

• [Journal Article] Natural variation in the genes responsible for maturity loci, E1, E2, E3 and E4 in soybean (2013)
  • Author(s): Tsubokura Y, Watanabe S, Xia Z, Kanamori H, Yamagata H, Kaga A, Katayose Y, Abe J, Ishimoto M, Harada K
  • Journal Title: Annals of Botany Volume: 113 Pages: 429-441
  • Peer Reviewed
• [Journal Article] Genetic variation in four maturity genes affects photoperiod insensitivity and PHYA-regulated post-flowering responses of soybean (2013)
  • Author(s): Xu M, Z. Xu, B. Liu, F. Kong, Y. Tsubokura, S. Watanabe, Z. Xia, K. Harada, A. Kanazawa, T. Yamada and J. Abe
  • Journal Title: BMC Plant Biology Volume: 11 Pages: 152
  • Peer Reviewed
• [Journal Article] Genetic variation in soybean at the maturity locus E4 Is involved in adaptation to long days at high latitudes (2013)
  • Author(s): Tsubokura Y, H. Matsumura, M. Xu, B. Liu, H. Kanamori, Y. Katayose, R. Takahashi, K. Harada and J. Abe
  • Journal Title: Agronomy Volume: 3 Pages: 117-134
  • Peer Reviewed
• [Journal Article] The National BioResource project (NBRP) Lotus and Glycine in Japan (2012)
  • Author(s): Hashiguchi M., J. Abe, T. Aoki, T. Anai, A. Suzuki and R. Akashi
  • Journal Title: Breeding Science Volume: 61 Pages: 531-543
  • Peer Reviewed
• [Journal Article] Genetic and molecular bases of photoperiod responses of flowering in soybean (2012)
  • Author(s): Watanabe S., K. Harada and J. Abe
  • Journal Title: Breeding Science Volume: 61 Pages: 453-461
  • Peer Reviewed
• [Presentation] ダイズの開花抑制遺伝子 E1 に制御される開花関連遺伝子の解析と開花との関連性 (2014)
  • Author(s): 竹島亮馬, 趙晨, 徐美蘭, 劉宝輝, 渡邊啓史, 山田哲也, 阿部純
  • Organizer: 日本育種学会第125回講演会
  • Place of Presentation: 東北大学,仙台市
  • Year and Date: 20140321-22
• [Presentation] VIGS を用いた E1 様遺伝子の機能解析 (2013)
  • Author(s): 徐美蘭, 山岸紀子, 竹島亮馬, 河西めぐみ, 金澤章, 吉川信幸, 阿部純
  • Organizer: 日本育種学会第124回講演会
  • Place of Presentation: 鹿児島大学,鹿児島市
  • Year and Date: 20131012-13
• [Presentation] 中国早生ダイズの非感光性に関する QTL 解析 (2013)
  • Author(s): 竹島亮馬, 趙晨, 徐美蘭, 劉宝輝, 渡邊啓史, 山田哲也, 阿部純
  • Organizer: 日本育種学会第123回講演会
  • Place of Presentation: 東京農業大学,東京都
  • Year and Date: 20130327-28
• [Presentation] Novel variation of photoperiod-insensitivity in soybean as revealed by genotyping of four major maturity locus with DNA markers (2012)
  • Author(s): Xu M,, Z. Xu, S. Watanabe, Y. Tsubokura, K. Harada, X. Yuan, Z. Xia, B. Liu, F. Kong, T. Yamada and J. Abe
  • Organizer: 10 th, International Congress on Plant Molecular Biology
  • Place of Presentation: Jeju Island, Republic of Korea
  • Year and Date: 20121021-26
• [Presentation] DNA 多型を利用したダイズ早生品種の感光性遺伝子型の分類と開花および開花後の感光性の変異 (2012)
  • Author(s): 徐美蘭, 徐沢恒, 坪倉康隆, 渡辺啓史, 夏正俊, 孔凡江, 劉宝輝, 原田久也, 山田哲也, 阿部純
  • Organizer: 日本育種学会第122回講演会
  • Place of Presentation: 都産業大学,京都
  • Year and Date: 20120914-15