Analysis of temperature environment and plant response

2013 Fiscal Year Final Research Report

• Project/Area Number: 23380003
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Breeding science
• Research Institution: Saitama University
• Principal Investigator: UCHIMIYA Hirofumi 埼玉大学, 環境科学研究センター, 教授 (50142229)
• Project Period (FY): 2011-11-18 – 2014-03-31
• Keywords: 温暖化 / 二酸化炭素 / 酸性土壌 / メタボローム解析 / ゲノム解析

Research Abstract

This study was conducted on the analysis of temperature effects in relation to the plant metabolic pathway and stress response. With several plant species metabolites and genome analysis was performed. Multiple analytical methods were used to evaluate variations of amino acids, organic acids, nucleotides and related compounds. Further global warming and related factors such as carbon dioxide, acidic soil and metal ions which may affect metabolite levels in plants were pursued.

Research Products

• [Journal Article] Effects of NADK2 overexpression on primary metabolite profiles in rice (2014)
  • Author(s): Onda, Y., Takahara, K., Miyagi, A., Uchimiya, H. and Kawai-Yamada, M
  • Journal Title: Plant Biology Volume: (in press)
  • Peer Reviewed
• [Journal Article] Culture temperature affects gene expression and metabolic pathways in the 2-methylisoborneolproducing cyanobacterium Pseudanabaena galeata (2014)
  • Author(s): Kakimoto, M., Ishikawa, T., Miyagi, A., Saito, K., Miyazaki, M., Asaeda, T., Yamaguchi, M., Uchimiya, H. and Kawai-Yamada, M
  • Journal Title: Journal of Plant Physiology Volume: 171 Pages: 292-300
  • Peer Reviewed
• [Journal Article] Metabolome analysis of food-chain between plants and insects (2013)
  • Author(s): Miyagi, A., Kawai-Yamada, M., Uchimiya, M., Ojima, N., Suzuki, K. and Uchimiya, H
  • Journal Title: Metabolomics Volume: 9 Pages: 1254-1261
  • Peer Reviewed
• [Journal Article] An antagonist treatment in combination with tracerexperiments revealed isocitrate pathway dominant to oxalate biosynthesis in Rumex obtusifolius L (2013)
  • Author(s): Miyagi, A., Uchimiya, M., Kawai- Yamada, M. and Uchimiya, H
  • Journal Title: Metabolomics Volume: 9 Pages: 590-598
  • Peer Reviewed
• [Journal Article] NAD+ accumulation as a metabolic off switch for orthodox pollen (2013)
  • Author(s): Hashida, S. N., Kawai-Yamada, M., and Uchimiya, H
  • Journal Title: Plant Signaling & Behavior Volume: 8 Pages: e23937.1-3
  • Peer Reviewed
• [Journal Article] The role of plant Bax Inhibitor-1 in suppressing H2O2-induced cell death (2013)
  • Author(s): Ishikawa, T., Uchimiya, H., and KawaiYamada, M
  • Journal Title: In Lester Packer, editors : Hydrogen Peroxide and cell signaling, Part B Volume: 527 Pages: 239-256
  • Peer Reviewed
• [Journal Article] Plastidic protein Cdf1 is essential in Arabidopsis embryogenesis (2013)
  • Author(s): Kawai-Yamada, M., Nagano, M., Kakimoto, M., and Uchimiya, H
  • Journal Title: Planta Volume: 239 Pages: 39-46
  • Peer Reviewed
• [Journal Article] Deletion of the transcriptional regulator cyAbrB2 de-regulates primary carbon metabolism in Synechocystis sp. PCC 6803 (2013)
  • Author(s): Kaniya, Y., Kisawa, A., Miyagi, A., Kawai-Yamada, M., Uchimiya, H., Kaneko, Y., Nishiyama, Y. and Hihara, Y
  • Journal Title: Plant Phys Volume: 162 Pages: 1153-1163
  • Peer Reviewed
• [Journal Article] Impact of aluminium stress in oxalate and other metabolites in Rumex obtusifolius (2013)
  • Author(s): Miyagi, A., Uchimiya, M., Kawai- Yamada, M. and Uchimiya, H
  • Journal Title: Weed Research Volume: 53 Pages: 30-41
  • Peer Reviewed
• [Journal Article] Plant sphingolipid fatty acid 2-hydroxylases have unique characters unlike their animal and fungus counterparts (2012)
  • Author(s): Nagano, M., Uchimiya, H., KawaiYamada, M
  • Journal Title: Plant Signaling & Behavior Volume: 7 Pages: 1388-1392
  • Peer Reviewed
• [Journal Article] NAD+ accumulation during pollen maturation in Arabidopsis regulating onset of germination (2012)
  • Author(s): Hashida, S.N., Takahashi, H., Takahara, K., Kawai-Yamada, M., Kitazaki, K, Shoji, K., Goto, F., Yoshihara, T., Uchimiya, H
  • Journal Title: Molecular Plant Volume: 6 Pages: 216-225
  • Peer Reviewed
• [Journal Article] Arabidopsis sphingolipid fatty acid 2-hydroxylases (AtFAH1 and AtFAH2) are functionally differentiated in fatty acid 2-hydroxylation and stress responses (2012)
  • Author(s): Nagano, M., Takahara, K., Fujimoto, M., Tsutsumi, N., Uchimiya, H., Kawai- Yamada, M
  • Journal Title: Plant Phys Volume: 159 Pages: 1138-1148
  • Peer Reviewed
• [Journal Article] Small acidic protein 1 acts with RUB modification components, the COP9 signalosome and AXR1, to regulate growth and development of Arabidopsis thaliana (2012)
  • Author(s): Nakasone, A., Fujiwara, M., Fukao, Y., Biswas, K.K., Rahman, A., Kawai-Yamada, M., Narumi, I., Uchimiya, H., Oono, Y
  • Journal Title: Plant Phys Volume: 163 Pages: 93-105
  • Peer Reviewed
• [Journal Article] The Gsp1 triggers sexual developmental program including inheritance of chloroplast DNA and mitochondrial DNA in Chlamydomonas reinhardtii (2012)
  • Author(s): Nishimura, Y., Shikanai, T., Nakamura, S., Kawai-Yamada, M., Uchimiya, H
  • Journal Title: Plant Cell Volume: 24 Pages: 2401-2414
  • Peer Reviewed
• [Journal Article] Nitrate addition alleviates ammonium toxicity without lessening ammonium accumulation, organic acid depletion and inorganic sation depletion in Arabidopsis thaliana shoots (2012)
  • Author(s): Hachiya, T., Watanabe, C.K., Fujimoto, M., Ishikawa, T., Takahara, K., Kawai-Yamada, M., Uchimiya, H., Uesono, Y., Terashima, I., Noguchi, K
  • Journal Title: Plant Cell Physiol Volume: 53 Pages: 577-591
  • Peer Reviewed
• [Journal Article] Molecular distinction in genetic regulation of nonphotochemical quenching in rice (2011)
  • Author(s): Kasajima, I., Ebana, K., Yamamoto, T., Takahara, K., Yano, M., Kawai-Yamada, M., Uchimiya, H
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 108 Pages: 13835-13840
  • Peer Reviewed
• [Journal Article] Fate of 13C in metabolic pathways and effects of High CO2 on the alteration of metabolites in Rumex obtusifolius L (2011)
  • Author(s): Miyagi, A., Takahara, K., Kasajima, I., Takahashi, H., Kawai-Yamada, M. and Uchimiya, H
  • Journal Title: Metabolomics Volume: 7 Pages: 524-535
  • Peer Reviewed
• [Presentation] Metabolome analysis of soluble oxalate accumulation in Rumex obtusifolius L (2014)
  • Author(s): Miyagi, A., Uchimiya, H. and KawaiYamada. M
  • Organizer: Plant Gene Discovery & "Omics" Technologies
  • Place of Presentation: Wien, Austria
  • Year and Date: 20140217-18
• [Presentation] Metabolomic improvement of NAD(P)(H) biosynthesis in plants (2014)
  • Author(s): Kawai-Yamada, M., Sato, Y. and Uchimiya. H
  • Organizer: Plant Gene Discovery & "Omics" Technologies
  • Place of Presentation: Wien, Austria
  • Year and Date: 20140217-18
• [Presentation] Functional association of cell death suppressor Arabidopsis Bax Inhibitor-1 with lipid metabolism (2013)
  • Author(s): Kawai-Yamada, M., Ishikawa, T., Nagano, M., Nakasone A. and Uchimiya, H
  • Organizer: 2013 Plant Symposium of the SEB, Oxidative stress and cell death in plants : mechanisms and implications
  • Place of Presentation: Florence, Italy
  • Year and Date: 20130726-28
• [Presentation] ガンマ線がエゾノギシギシのシュウ酸代謝に及ぼす影響 (2013)
  • Author(s): 北野沙也佳, 宮城敦子, 川合真紀, 内宮博文, 山口雅利, 大野豊, 長谷純宏, 鳴海一成
  • Organizer: 第8回高崎量子応用研究シンポジウム
  • Place of Presentation: 高崎
  • Year and Date: 2013-10-11
• [Presentation] 植物におけるシュウ酸蓄積機構のメタボローム解析 (2013)
  • Author(s): 宮城敦子, 内宮博文, 川合真紀
  • Organizer: 第8回メタボロームシンポジウム
  • Place of Presentation: 福岡
  • Year and Date: 2013-10-03
• [Presentation] Δ8スフィンゴ脂質不飽和化酵素の酵素活性に関わる領域の検索 (2013)
  • Author(s): 中曽根光, 石川寿樹, 内宮博文, 川合真紀
  • Organizer: 第26回植物脂質シンポジウム
  • Place of Presentation: 札幌
  • Year and Date: 2013-09-16
• [Presentation] シロイヌナズナの脂肪酸伸長酵素の蒸散制御への寄与 (2013)
  • Author(s): 柿沼悠太, 石川寿樹, 長野稔, 山口雅利, 内宮博文, 川合真紀
  • Organizer: 第26回植物脂質シンポジウム
  • Place of Presentation: 札幌
  • Year and Date: 2013-09-16
• [Presentation] シロイヌナズナの低温ストレス応答におけるスフィンゴリピドーム解析 (2013)
  • Author(s): 石川寿樹, 長野稔, 中曽根光, 内宮博文, 川合真紀
  • Organizer: 第26回植物脂質シンポジウム
  • Place of Presentation: 札幌
  • Year and Date: 2013-09-15
• [Presentation] Al3+存在下における高シュウ酸植物エゾノギシギシのメタボローム解析 (2013)
  • Author(s): 宮城敦子, 内宮博文, 川合真紀
  • Organizer: 第78回日本植物学会
  • Place of Presentation: 札幌
  • Year and Date: 2013-09-14
• [Presentation] ガンマ線が高シュウ酸植物の代謝に及ぼす影響 (2013)
  • Author(s): 北野沙也佳, 宮城敦子, 大野豊, 長谷純弘, 鳴海一成, 山口雅利, 内宮博文, 川合真紀
  • Organizer: 第78回日本植物学会
  • Place of Presentation: 札幌
  • Year and Date: 2013-09-14
• [Presentation] 蒸散制御におけるシロイヌナズナの脂肪酸伸長酵素 AtELO1 の機能解析 (2013)
  • Author(s): 柿沼悠太, 石川寿樹, 長野稔, 山口雅利, 内宮博文, 川合真紀
  • Organizer: 第78回日本植物学会
  • Place of Presentation: 札幌
  • Year and Date: 2013-09-13
• [Presentation] 出芽酵母発現系を用いたスフィンゴ脂質長鎖塩基Δ8位不飽和化酵素のドメイン解析 (2013)
  • Author(s): 中曽根光, 石川寿樹, 内宮博文, 川合真紀
  • Organizer: 第78回日本植物学会
  • Place of Presentation: 札幌
  • Year and Date: 2013-09-13
• [Presentation] シロイヌナズナの低温応答におけるスフィンゴ脂質代謝動態 (2013)
  • Author(s): 石川寿樹, 中曽根光, 長野稔, 内宮博文, 川合真紀
  • Organizer: 第78回日本植物学会
  • Place of Presentation: 札幌
  • Year and Date: 2013-09-13
• [Presentation] シュウ酸合成阻害エゾノギシギシのメタボローム解析 (2013)
  • Author(s): 宮城敦子, 内宮博文, 川合真紀
  • Organizer: 第31回日本植物細胞分子生物学会
  • Place of Presentation: 札幌
  • Year and Date: 2013-09-11
• [Presentation] Molecular analaysis of Arabidopsis Δ8-sphingolipid desat urase under cold stress (2011)
  • Author(s): Iwabuchi, M., Nagano, M., Ishikawa, T., Yamaoka, Y., Nishida, I., Uchimiya, H. and Kawai-Yamada, M
  • Organizer: The 4th Asian Symposium on Plant Lipids
  • Place of Presentation: Hong Cong, China
  • Year and Date: 2011-12-03
• [Presentation] Proteome analysis to identify membrane microdomain proteins involved in regulation of stress-induced cell death (2011)
  • Author(s): Kawai-Yamada, M., Ishikawa, T., Nagano, M., Aki, T., Yanagisawa, S. and Uchimiya, H
  • Organizer: 3rd International Symposium on Frontiers in Agriculture Proteome Research
  • Place of Presentation: Tsukuba, Japan
  • Year and Date: 2011-11-08
• [Presentation] バイオマス資源雑草のメタボローム解析 (2011)
  • Author(s): 宮城敦子, 川合真紀, 内宮博文
  • Organizer: 第6回メタボロームシンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2011-10-14
• [Presentation] 植物のスフィンゴ脂質脂肪酸 2-ヒドロキシラーゼの解析 (2011)
  • Author(s): 長野稔, 石川寿樹, 内宮博文, 川合真紀
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-24
• [Presentation] Bax inhibitor-1 による細胞死制御機構に関与するイネ長鎖塩基Δ4 不飽和化酵素の解析 (2011)
  • Author(s): 石川寿樹, 内宮博文, 川合真紀
  • Organizer: 日本植物脂質科学研究会
  • Place of Presentation: 東京
  • Year and Date: 2011-09-20
• [Presentation] Bax Inhibitor-1 の酸化ストレス耐性機構に関与する細胞膜マイクロドメインタンパク (2011)
  • Author(s): 石川寿樹, 秋利彦, 柳澤修一, 長野 稔, 内宮博文, 川合真紀
  • Organizer: 第76回日本植物学会
  • Place of Presentation: 東京
  • Year and Date: 2011-09-17
• [Presentation] シロイヌナズナΔ8-スフィンゴ脂質不飽和化酵素の低温応答における機能 (2011)
  • Author(s): 岩渕充, 長野稔, 石川寿樹, 内宮博文, 川合真紀
  • Organizer: 第76回日本植物学会
  • Place of Presentation: 東京
  • Year and Date: 2011-09-17
• [Presentation] 細胞内還元力プールの改変による植物バイオマス制御 (2011)
  • Author(s): 川合真紀, 高原健太郎, 恩田弥生, 内宮博文
  • Organizer: 植物細胞分子生物学会シンポジウム
  • Place of Presentation: 福岡
  • Year and Date: 2011-09-07
• [Presentation] 雑草のバイオマス生産制御とメタボローム解析 (2011)
  • Author(s): 宮城敦子, 川合真紀, 内宮博文
  • Organizer: 植物細胞分子生物学会
  • Place of Presentation: 福岡
  • Year and Date: 2011-09-07
• [Patent(Industrial Property Rights)] シュウ酸濃度が低減された植物体の製造法 (2013)
  • Inventor(s): 大野豊, 鳴海一成, 長谷純弘, 川合真紀, 北野沙也佳, 宮城敦子, 内宮博文
  • Industrial Property Rights Holder: 大野豊, 鳴海一成, 長谷純弘, 川合真紀, 北野沙也佳, 宮城敦子, 内宮博文
  • Industrial Property Number: 特願2013-023290
  • Filing Date: 2013-02-28