Establishment of a new breeding procedure based on rice gene targeting using information of molecular genetics and attempt to generate a new variety.

2013 Fiscal Year Final Research Report

• Project/Area Number: 23380008
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Breeding science
• Research Institution: Meijo University
• Principal Investigator: TERADA Rie 名城大学, 農学部, 教授 (30137799)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: 植物分子育種学 / 遺伝子ターゲティング / イネ / ポジティブ・ネガティブ選抜

Research Abstract

For generation of a rice new variety by gene targeting (GT) procedure, the OsRac1 was modified by GT based on positive-negative selection to generate blast fungus resistance rice. Since the G19V substitution in OsRac1 had shown the effective immunity in random transgenic rice, the same modification at the original OsRac1 locus was examined. Following to GT for G19V substitution positive-marker remained in the OsRac1 locus was removed by Cre-loxP mediated marker free system. The OsRac1 activation was detected in the gene-modified rice plants of segregated homozygous lines for the G to T alternation in the exon 1, however, unexpected growth inhibition in the plants was also associated. For generation of arsenic resistant rice, the 16 of rice plant lines having PvACR were obtained.

Research Products

• [Journal Article] The MET1b gene encoding a maintenance DNA methyltransferase is indispensable for normal development in rice (2014)
  • Author(s): Yamauchi T, Johzuka-Hisatomi Y, Terada R, Nakamura I, Iida S
  • Journal Title: Plant Mol Biol Volume: (in press)
  • DOI: 10.1007/s11103-014-0178-9
• [Journal Article] Gene editing a constitutively active OsRac1 by homologous recombination-based gene targeting induces immune responses in rice (2013)
  • Author(s): Dang TT, Shimatani Z, Kawano Y, Terada R, Shimamoto K
  • Journal Title: Plant Cell Physiology Volume: 54 Pages: 2058-2070
  • DOI: 10.1093/pcp/pct147
• [Journal Article] A null mutation of ROS1a for DNA demethylation in rice is not transmittable to progeny (2012)
  • Author(s): Ono, A., Yamaguchi, K., Fukada-Tanaka, S., Terada, R., Mitsui, T., Iida, S
  • Journal Title: The Plant Journal Volume: 71 Pages: 564-574
  • DOI: 10.1111/j.1365-313X.2012.05009.x
• [Journal Article] Targeted disruption of an orthologue of DOMAINS REARRANGED METHYLASE2, OsDRM2, impairs the growth of rice plants by abnormal DNA methylation (2012)
  • Author(s): Moritoh, S., Eun, C-H, Ono, A., Asao, H., Okano, Y., Yamaguchi, K., Shimatani, Z., Koizumi, A. and Terada R
  • Journal Title: The Plant Journal Volume: 71 Pages: 85- 98
  • DOI: 10.1111/j.1365-313X.2012.04974.x
• [Presentation] 植物ゲノム編集技術としての遺伝子ターゲティング : 技術の現状とイネ耐病性関連遺伝子 OsRac1 の恒常活性化改変 (2014)
  • Author(s): 島谷 善平, 寺田 理枝, Thu Thi Dang, 河野 洋治, 辻 寛之, 田岡 健一郎, 島本功
  • Organizer: 本育種学会第125回講演会;平成26年春季大会
  • Place of Presentation: 東北大学
  • Year and Date: 2014-03-21
• [Presentation] 島本功ジーンターゲティングにより作成した OsMADS15-mOrange トランスジェニックイネの解析 (2012)
  • Author(s): 松本彩奈, 島谷善平, 辻寛之, 寺田理枝
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡
  • Year and Date: 2012-12-12
• [Presentation] 日遺伝子機能解析と分子育種を推進するイネの遺伝子ターゲティング : 相同組換えが作り出す様々な変異イネ (2011)
  • Author(s): 島谷善平・寺田理枝
  • Organizer: 日本遺伝学会第83回大会ワークショップ
  • Place of Presentation: 京都大学
  • Year and Date: 2011-09-24
• [Book] バイオサイエンス アドバンスドマニュアルシリーズ「形質転換プロトコル(植物編) 」トピックス; イネのターゲッティング・ プロトコル (2012)
  • Author(s): 土岐精一 ・ 寺田理枝 ・ 島谷善平 ・ 横井彩子・刑部敬史
  • Total Pages: 364-371
  • Publisher: 化学同人
• [Patent(Industrial Property Rights)] 植物体に用いる組換えベクター及びその利用 (2014)
  • Inventor(s): 寺田理枝・島谷善平
  • Industrial Property Rights Holder: 学校法人名城大学
  • Industrial Property Number: 特願2014-25910号
  • Filing Date: 2014-02-13