Molecular analysis of appearing mechanism of aposporous embryo sac initial cell in Panicum maximum

2014 Fiscal Year Final Research Report

• Project/Area Number: 23380009
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Science in genetics and breeding
• Research Institution: Minami Kyusyu University
• Principal Investigator: CHEN Lanzhuang 南九州大学, 環境園芸学部, 教授 (40284822)
• Co-Investigator(Kenkyū-buntansha): 吉田 薫 東京大学, 農学部 (70183994) ; 鉄村 琢哉 宮崎大学, 農学部 (00227498)
• Co-Investigator(Renkei-kenkyūsha): 東山 哲也 名古屋大学, 理学部 (00313205) ; 野々村 賢一 国立遺伝学研究所 (10291890) ; 剣持 直哉 宮崎大学, フロンティア科学実験総合センター (00133124)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Keywords: アポミクシス / 胚嚢始原生殖細胞 / ASG-1遺伝子の機能解析 / ギニアグラス / シロイヌナズナ / イネ組換え植物 / 多胚嚢形成現象 / 多胚形成現象

Outline of Final Research Achievements

For the molecular analysis of appearing mechanism of aposporous embryo sac initial cell (AIC) in apomictic guinea grass in this study, 1) the AIC was isolated after ovaries treated with enzyme solution and by this its existence was proofed; 2) the apomixis specific gene (ASG-1) isolated from apomict of guinea grass (showing multiple embryo sac formation (MES)) was used for plasmid construction of hsp::ASG-1::GFP to produce transformants of Arabidopsis. In T4∼T5 generations,1) GFP expression was observed in each organism; 2) in experiments of either rapid or long time drought treatment, the plants showed drought resistance comparing to the non-transformants; 3) the following phenomena in the same ovule were observed in part of the plants (20%): a) MES formation; b) multiple embryogenesis (ME) (one embryo and the embryoid initiated from micropylar end); c) ME (one embryo from micropylar end, and the embryoid from the chalazal end).

Free Research Field

遺伝育種学