Studies on MADS-box transcription factors associated with bud dormancy regulation in the fruit tree species of Prunus

2014 Fiscal Year Final Research Report

• Project/Area Number: 23380017
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Horticulture/Landscape architecture
• Research Institution: Kyoto University
• Principal Investigator: YAMANE Hisayo 京都大学, (連合)農学研究科(研究院), 講師 (80335306)
• Co-Investigator(Kenkyū-buntansha): TAO Ryutaro 京都大学, 大学院農学研究科, 准教授 (10211997) ; HABU Tsuyoshi 愛媛大学, 農学部, 准教授 (60335304)
• Research Collaborator: AKAGI Takashi ; NAGAYAMA Erika ; HOSAKA Yukari ; SASAKI Ryuta ; TAKEUCHI Nozomi
• Project Period (FY): 2011-04-01 – 2015-03-31
• Keywords: 果樹 / 休眠 / 転写因子

Outline of Final Research Achievements

Bud dormancy of temperate fruit trees is one of the important agronomic traits that affects next season’s annual growth. In this study, we used 454-pyrosequencing technique to gather sequence information of dormant bud ESTs in Prunus mume and to search for the candidate genes that are involved in endodormancy regulation. As a result, PmDAM4 and PmDAM5 were found to be up-regulated in endodormant buds as is the case with PmDAM6 which has already been identified as endodormancy-related gene in our previous studies. All six PmDAM genes were up-regulated when chilling exposure was avoided and down-regulated during cold treatment. Among them, PmDAM4, PmDAM5, and PmDAM6 were down-regulated by cold treatment in chilling requirement-dependent manner. These results suggested that PmDAMs play roles in genetic control of chilling requirement of Prunus mume.

Free Research Field

園芸学