Development of pollen S-specificity in self-incompatibility in Japanese pear

2014 Fiscal Year Final Research Report

• Project/Area Number: 23380018
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Horticultural science
• Research Institution: Kobe University
• Principal Investigator: TAKASAKI-YASUDA TAKESHI 神戸大学, (連合)農学研究科(研究院), 教授 (30314511)
• Co-Investigator(Kenkyū-buntansha): ISHIMIZU Takeshi 立命館大学, 生命科学部, 准教授 (30314355)
• Co-Investigator(Renkei-kenkyūsha): ISHIMIZU Takeshi 立命館大学, 生命科学部, 准教授 (30314355)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Keywords: 自家不和合性 / ナシ / 果樹園芸学 / 分子遺伝学 / 細胞組織学

Outline of Final Research Achievements

Self-incompatibility in Japanese pear is controlled by S-haplotype at the S-locus, which code a pistil S determinant, S-RNase, and pollen S determinants, S-locus F-box brothers (PpSFBBs). In this study, we expanded BAC contigs around S-RNase to find at least 17 PpSFBB2s coded by S2-haplotype. PpSFBB2 homologs cloned from pollen of S homozygous lines had high identity between haplotypes, which predicts that the collaborative non-self S-RNase recognition model in Solanaceae species fits the S-RNase-based SI systems in the Japanese pear. Using the immune microscopy, we confirmed endocytic uptake of S-RNase into pollen tube, and subsequent trafficking via endosomes.

Free Research Field

農学