2013 Fiscal Year Final Research Report
Genome wide analysis for genes involved in the lepidopteran insect sex determination cascade and its application to pest control
| Project/Area Number |
23380032
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied entomology
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
SUZUKI Masataka 東京大学, 新領域創成科学研究科, 准教授 (30360572)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
AOKI Fugaku 東京大学, 大学院・新領域創成科学研究科, 教授 (20175160)
|
|---|
| Project Period (FY) |
2011-04-01 – 2014-03-31
|
| Keywords | 性決定 / 性分化 / 性決定遺伝子 / 選択的スプライシング / de novo トランスクリプトーム / カイコ / 害虫防除 / W染色体 |
|---|
| Research Abstract |
We found that a Bombyx homolog of tra-2 is dispensable for the sex-specific splicing of Bmdsx pre-mRNA. Instead, a male-specific splice variant of Imp is involved in the male-specific splicing of Bmdsx. The male-specific expression of Imp is controlled by its autoregulatory function, whereby the protein product regulates the male-specific splicing of its own pre-mRNA. This suggests that Imp acts as the memory device for sex determination in the silkworm. In an attempt to identify the Fem gene of B. mori, we performed comparative analysis of de novo transcriptome assembly between male and female embryos at the sex-determining stage. Among 149 cDNAs that were expressed at more than eight-fold higher in females than in males, two cDNAs (FET-W1, FET-W2) were derived from genes located on the sex-determining region of the W chromosome. Knockdown of FETW-1 induced expression of male-type Bmdsx and ImpM in female eggs. These results suggest that FET-W1 is the most likely candidate for Fem.
|
-
[Journal Article] A single female-specific piRNA is the primary determiner of sex in the silkworm2014
Author(s)
Kiuchi T ., Koga H., Kawamoto M., Shoji K., Sakai H., Arai Y ., Ishihara G ., Kawaoka S., Sugano S., Shimada T ., Suzuki Y ., Suzuki M. G ., and Katsuma S
-
Journal Title
Nature
Volume: (in press)
Peer Reviewed
-
-
[Journal Article] Genome-wide analysis of the chromatin composition of histone H2A and H3 variants in mouse embryonic stem cells2014
Author(s)
Yukawa M., Akiyama T., Franke V., Mise N., Isagawa T., Suzuki Y., Suzuki M. G ., Vlahovicek K., Abe K., Aburatani H., Aoki F
-
Journal Title
PLoS One
Volume: 9
Pages: e92689.
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
