2013 Fiscal Year Final Research Report
Degradation of rRNA in insect cells antiviral immune response
| Project/Area Number |
23380033
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | Nagoya University |
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Principal Investigator |
IKEDA Motoko 名古屋大学, 生命農学研究科, 教授 (20262892)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 核多角体病ウイルス / 昆虫細胞 / 抗ウイルス応答 / リボソームRNA分解 / 全タンパク質合成停止 / カイコ |
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| Research Abstract |
Previously, we demonstrated that rRNA degradation occurs in Bombyx cells (BM-N cells) during abortive infection with heterologous nucleopolyhedroviruses (NPVs). rRNA degradation is likely linked to the global protein synthesis shutdown observed in BM-N cells infected with heterologous NPVs. In the present study, we examined the molecular mechanisms of rRNA degradation of BM-N cells. We cloned cDNAs of RNase and RNase inhibitor from BM-N cells possibly responsible for rRNA degradation, and functional analyses were performed by the transient gene expression and RNAi-based gene silencing. We also examined the possible Bombyx mori NPV factor(s) responsible for inhibition of rRNA degradation in BM-N cells.
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[Journal Article] Degradation of ribosomal RNA in BM-N cells from the silkworm Bombyx mori during abortive infection with heterologous nucleopolyhedroviruses2013
Author(s)
Hamajima, R., Ito, Y., Ichikawa, H., Mitsutake, H., Kobyashi, J., Kobayashi, M. and Ikeda, M
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Journal Title
J. Gen. Virol
Volume: 94
Pages: 2102-2111
DOI
Peer Reviewed
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[Journal Article] Cloning and characterization of a dronc homologue in the silkworm, Bombyx mori2011
Author(s)
Suganuma, I., Ushiyama, T., Yamada, H., Iwamoto, A., Kobayashi, M. and Ikeda, M
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Journal Title
Insect. Biochem. Mol. Biol
Volume: 41
Pages: 909-921
DOI
Peer Reviewed
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