Making of multi-tolerant plants against complexed acid-soil stresses by molecular modifications of membrane lipid layers

2013 Fiscal Year Final Research Report

• Project/Area Number: 23380041
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Yamagata University
• Principal Investigator: WAGATSUMA Tadao 山形大学, 農学部, 客員教授 (70007079)
• Co-Investigator(Kenkyū-buntansha): TAWARAYA Keitarou 山形大学, 農学部, 教授 (70179919) ; WASAKI Jun 広島大学, 生物圏科学研究科, 准教授 (00374728) ; WATANABE Toshihiro 北海道大学, (連合) 農学研究科 (研究院), 准教授 (60360939)
• Co-Investigator(Renkei-kenkyūsha): KOYAMA Hiroyuki 岐阜大学, 応用生物科学部, 教授 (90234921) ; TOYOMASU Tomonobu 山形大学, 農学部, 教授 (60272085) ; KURODA Masaharu 独立行政法人農業, 食品産業技術総合研究機構・中央農業総合研究センター稲収量性研究北陸サブチーム, 主任研究員 (30355581) ; OIKAWA Akira 山形大学, 農学部, 准教授 (50442934)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: アルミニウム耐性 / ガラクト脂質 / ステロール / 低リン耐性 / メタボローム / リン脂質 / リンリサイクリング能

Research Abstract

In order to lower the content of phospholipids and to increase that of sterols in roots, we made the new plants by modification using two different techniques, i.e., culturing method and molecular method: Al-sensitive rice cultivar Koshihikari grown previously under P-deficient conditions and transformed by the overexpressions of PAH (key gene to exchange phospholipids into galactolipids) and HMG (key gene to biosynthesize sterols). Al tolerance and low-Ca tolerance under low pH conditions of P-deficient Al-sensitive Koshihikari were significantly enhanced, and Al tolerance and low-P tolerance of the several lines of the transformed Koshihikari were also enhanced. Among several tens of rice cultivars, low-P tolerant rice cultivar was found out to have a specific characteristics on the decomposition of P-containing metabolites. Several genes contributing to P-recycling ability were identified together with each function.

Research Products

• [Journal Article] Phosphorus deficiency enhances aluminum tolerance of rice (Oryza sativa) by changing the physicochemical characteristics of root plasma membranes and cell walls (2014)
  • Author(s): Maejima, E., Watanabe, T., Osaki, M. and Wagatsuma, T
  • Journal Title: J Plant Physiol Volume: 171 Pages: 9-15
  • DOI: 10.1016/j.jplph.2013.09.012
  • Peer Reviewed
• [Journal Article] Molecular and physiological analysis of Al^<3+> and H^<+> rhizotoxicities at moderately acidic conditions (2013)
  • Author(s): Kobayashi, Y., Kobayashi, Y., Watanabe, T., Shaf J. E., Ohta, H., Kochian, L. V., Wagatsuma, T., Kinraide, T. B., Koyama, H
  • Journal Title: Plant Physiol Volume: 163 Pages: 180-192
  • DOI: 10.1104/pp.113.222893
  • Peer Reviewed
• [Journal Article] Metabolite profiling of shoot extracts, root extracts, and root exudates of rice plant under phosphorus deficiency (2013)
  • Author(s): Tawaraya, K., Horie, R., Saito, A., Shinano, T., Wagatsuma, T., Saito, K., Oikawa, A
  • Journal Title: J Plant Nutr Volume: 36 Pages: 1138-1159
  • DOI: 10.1080/01904167.2013.780613
  • Peer Reviewed
• [Journal Article] リン酸資源の枯渇に対応したリン栄養研究. 1.講座のねらい (2012)
  • Author(s): 俵谷 圭太郎, 和崎 淳
  • Journal Title: 日本土壌肥料学雑誌 Volume: 83 Pages: 173-176
  • Peer Reviewed
• [Journal Article] Proteomic analysis of secreted proteins from aseptically grown rice (2011)
  • Author(s): Shinano, T., Komatsu, S., Yoshimura, T., Tokutake, S., Kong, F.J., Watanabe, T., Wasaki, J., Osaki, M
  • Journal Title: Phytochem Volume: 72 Pages: 302-311
  • DOI: 10.1016/j.phytochem.2010.12.006
  • Peer Reviewed
• [Presentation] Phosphorus deficiency enhances the tolerance of rice to the acidic soil stress (2013)
  • Author(s): Maejima, E
  • Organizer: XVII International Plant Nutrition Colloquium 2013
  • Place of Presentation: Istanbul (Turkey)
  • Year and Date: 20130819-22
• [Presentation] HMG is a promising key gene for the enhancement of aluminum tolerance of rice via modulating membrane sterols in root-tip portion (2013)
  • Author(s): Wagatsuma, T
  • Organizer: XVII International Plant Nutrition Colloquium 2013
  • Place of Presentation: Istanbul (Turkey)
  • Year and Date: 20130819-22
• [Presentation] Comparative transcriptomic analysis of P deficient plant roots (2012)
  • Author(s): Wasaki, J
  • Organizer: Japan-Australia Symposium on Plant Sciences for Agriculture IV
  • Place of Presentation: Perth (Australia)
  • Year and Date: 20121211-12
• [Presentation] イネの根脂質改変とアルミニウム耐性 (2012)
  • Author(s): 前島恵理子
  • Organizer: 日本土壌肥料学会本大会2012年度鳥取大会
  • Place of Presentation: 鳥取大学(鳥取市)
  • Year and Date: 20120904-06
• [Presentation] Metabolite profiling of shoot extracts, root extracts, and root exudates of rice under phosphorus deficiency
  • Author(s): Tawaraya, K
  • Organizer: 8th Symposium of the International Society of Root Research
  • Place of Presentation: Dundee (UK)
• [Book] Rijeka, Croatia. Physiological and biochemical mechanisms of plant adaptation to low-fertility acid soils of the tropics: The case of Brachiariagrasses. In Principles, Application and Assessment in Soil Science (2011)
  • Author(s): Watanabe, T., Khan, M.S.H., Rao, I.M., Wasaki, J., Shinano, T., Ishitani, M., Koyama, K., Ishikawa, S., Tawaraya, K., Nanamori, M., Ueki, N., Wagatsuma T
  • Total Pages: 87-116
  • Publisher: Intech