2013 Fiscal Year Final Research Report
Transcription profiles of host plant genes induced in the rhizobial and arbuscular mycorrhizal symbioses of soybean root
| Project/Area Number |
23380042
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | Chiba University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
MATSUI Akihiro 独立行政法人理化学研究所, 環境資源科学研究センター, 特別研究員 (90443027)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | ダイズ / 根粒菌 / アーバスキュラー菌根菌 / 共生関係 / 遺伝子発現 / DNAマイクロアレイ |
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| Research Abstract |
In this study, the transcription profiles of host genes induced by rhizobial, arbuscular mycorrhizal and dual inoculations were analyzed to elucidate the host genes involving in the establishment of the dual symbiosis in soybean root. We found that 34 genes of host plant were induced commonly by rhizobial, mycorrhizal and dual inoculations, suggesting that these genes are essential for establishing dual symbiosis. The gene expression of type1 metallothionein and CPRD49 were examined using real-time RT-PCR. It was ascertained that the expression of type1 metallothionein gene was significantly induced in the root nodule and mycorrhizal root of soybean. We tried to examine the spatial expression patterns of type1 metallothionein gene in the transgenic hairy roots using promoter-gus gene fusions.
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Research Products
(13 results)
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[Journal Article] Complete genomic sequence of Bradyrhizobium sp. S23321 : insights into symbiosis evolution in soil oligotrophs2012
Author(s)
Okubo, T., Tsukui, T., Maita, H., Okamoto, S., 他20名, Sakamoto, K., 他24名
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Journal Title
Microbes and Environments
Volume: 27
Pages: 306-315
DOI
Peer Reviewed
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[Presentation] Transcription profiles of host plant genes induced by rhizobial, AM fungal and dual inoculations in soybean root2013
Author(s)
Sakamoto, K., Ogiwara, N., Kaji, T., Seki, M., Matsui, A., Ishida, J. and Tanaka, M
Organizer
18th International Congress on Nitrogen Fixation
Place of Presentation
Phoenix Seagaia Resort (Miyazaki city)
Year and Date
2013-10-16
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