2013 Fiscal Year Final Research Report
Studies on the novel cellular functions of phospholipases A2
| Project/Area Number |
23380047
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ARIOKA Manabu 東京大学, 農学生命科学研究科, 准教授 (20242159)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | phospholipase A2 / lysophosphatidylcholine / Aspergillus oryzae / nerve growth factor / MAPK |
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| Research Abstract |
(1) One of the secretory phospholipases A2 (PLA2s) in Aspergillus oryzae, sPlaB, displayed PLA1, not PLA2, activity.
(2) A cytosolic PLA2-like protein in A. oryzae, AoPlaA, displayed phosphatidylethanolamine-specific PLA2 activity. Alanine substitution of putative active-site residues resulted in the loss of PLA2 activity.
(3) Nerve growth factor (NGF)-induced mitogen-activated protein kinase (MAPK) phosphorylation in PC12 cells was found to be potentiated by the addition of lysophosphatidylcholine (LPC). Group X PLA2 displayed an effect similar to LPC. LPC augmented NGF-induced activation of TrkA, the receptor for NGF, thereby potentiating the downstream signaling pathways. By domain-swapping experiments, the extracellular domain of NGF was shown to be responsible for the effect of LPC.
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