2013 Fiscal Year Final Research Report
Elucidation of transcription network of stringent control in Bacillus subtilis and its application
| Project/Area Number |
23380053
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Fukuyama University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HIROOKA Kazutake 福山大学, 生命工学部, 准教授 (20389068)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 緊縮応答 / 枯草菌 / 発現制御 / 応用微生物 / ゲノム / 胞子形成 / 緊縮転写制御 / プリン合成 |
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| Research Abstract |
In Bacillus subtilis, stringent response evokes a GTP decrease and an ATP increase. As a result, positive and negative stringent transcription regulation of stringent operons possessing adenine and guanine in their transcription initiation sites, occur, respectively. Among positive stringent genes are kinA and kinB which are necessary for sporulation initiation. Operation of positive stringent transcription of kinA and kinB was found to be prerequisite for sporulation initiation.
As an approach to the applied field, guanine at the transcription initiation site of the pts operon under negative stringent control was converted to adenine, so that glucose metabolism was enhanced in the stringent conditions to result in more than 5-fold increase of isoleucine production by ilv-leu operon involved in branched-chain amino acid synthesis which is subjected to positive stringent transcription control.
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[Book] Creation of novel technologies for extracellular protein production toward the development of Bacillus subtilis genome factories pp. 3-15 in Microbial Production; From Genome Design to Cell Engineering2014
Author(s)
Katsutoshi Ara, Kenji Manabe, Shenghao Liu, Yasushi Kageyama, Tadahiro Ozawa, Masatoshi Tohata, Keiji Endo, Kazuhisa Sawada, Nozomu Shibata, Akihito Kawahara, Kazuhiro Saito, Hiroshi Kodama, Yoshiharu Kimura, Katsuya Ozaki, Yoshinori Takema, Hiroshi Kakeshita, Kouji Nakamura, Kunio Yamane, Takeko Kodama, Junichi Sekiguchi, Takuya Morimoto, Ryosuke Kadoya, Shigehiko Kanaya, Yasutaro Fujita, Fujio Kawamura, Naotake Ogasawara
Total Pages
306
Publisher
Springer Japan, Tokyo
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