2013 Fiscal Year Final Research Report
Novel function of ribosome involved in RsgA/RbfA
| Project/Area Number |
23380054
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Hirosaki University |
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Principal Investigator |
HYOUTA Himeno 弘前大学, 農学生命科学部, 教授 (80208785)
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| Co-Investigator(Kenkyū-buntansha) |
MUTO Akira 弘前大学, 農学生命科学部, 研究員 (80034635)
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| Co-Investigator(Renkei-kenkyūsha) |
TAKEMOTO Chie 独立行政法人理化学研究所, 生命分子システム基盤研究領域, 上級研究員 (40306527)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | RsgA / YjeQ / RbfA / リボソーム / GTPase / 浸透圧ストレス / RimM |
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| Research Abstract |
From analyzing RsgA-deficient Escherichia coli strain and its revertant strains, we found a function of RsgA having a GTPase activity: RsgA dissociates RbfA from the small (30S) subunit during the last stage of ribosome maturation. In addition, the roles of three ribosome maturation factors including RsgA, RbfA and RimM in the ribosome maturation process have been investigated using biochemical and structural approaches.
We also found that RsgA deprivation provides resistance to osmotic (salt) stress with the cell. Resistance to osmotic (salt) stress is also provided by deprivation of other ribosome maturation factors.
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[Journal Article] Structural insights into the assembly of the 30S ribosomal subunit in vivo: functional role of S5 and location of the 17S rRNA precursor sequence.2014
Author(s)
Yang, Z., Guo, Q., Goto, S., Chen, Y., Li, N., Yan, K., Zhang, Y., Muto, A., Deng, H., Himeno, H., Lei, J., Gao, N.
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Journal Title
Protein & Cell
Volume: Vol.5
Pages: 394-407
DOI
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[Journal Article] Dissecting the in vivo assembly of the 30S ribosomal subunit reveals the role of RimM and general features of the assembly process.2013
Author(s)
Guo, Q., Goto, S., Chen, Y., Feng, B., Xu, Y., Muto, A., Himeno, H., Deng, H., Lei, J., Gao, N.
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Journal Title
Nucleic Acids Res.
Volume: Vol.41
Pages: 2609-2620
DOI
Peer Reviewed
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[Presentation] Recognition of mRNA length on the ribosome by tmRNA andSmpB.2013
Author(s)
Kurita, D., Miller, M.R., Muto, A., Buskirk, A.R., Himeno, H.
Organizer
Ribosomes Conference 2013
Place of Presentation
Napa Valley, California
Year and Date
20130709-12
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[Presentation] The first translocation in trans-translation.2012
Author(s)
Takada, K., Kurita, D., Naoe, C., Yokogawa, T., Himeno, H., Takemoto, C.
Organizer
第14回日本RNA学会年会
Place of Presentation
仙台
Year and Date
20120718-20
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[Presentation]2011
Author(s)
後藤史門,武藤あきら,姫野俵太
Organizer
第34回日本分子生物学会
Place of Presentation
横浜
Year and Date
20111200
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