2014 Fiscal Year Final Research Report
High-Throughput Genetic Identification of Regulatory Factors Involved in the Regulation of Seed Oil Storage Using Bioluminescence Automatic Monitoring System
Project/Area Number |
23380057
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Applied biochemistry
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Research Institution | Chubu University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
ISHIURA Masahiro 名古屋大学, 遺伝子実験施設, 教授 (20132730)
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Project Period (FY) |
2011-04-01 – 2015-03-31
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Keywords | 種子成熟 / 種子発芽 / 油脂貯蔵 / 突然変異株 / 発光レポーター / 次世代シーケンサー |
Outline of Final Research Achievements |
We prepared Arabidopsis plants that express LUC reporter gene under the control of promoters from genes important in the biosynthesis and storage of seed oil. By using automatic bioluminescence monitoring system, we screened several hundred thousands of plants for mutants with altered expression patterns of LUC reporter gene. In addition to AOTA1, a novel factor involved in the activation of DGAT1 gene for the final step of seed oil synthesis, we could identify several additional candidate genes potentially involved in the activation of DGAT1. Furthermore, we could identify a novel gene DROL1 coding for a factor potentially involved in the repression of OleS3 gene for seed oil storage after seed germination.
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Free Research Field |
植物生化学・分子生物学
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