2013 Fiscal Year Final Research Report
Analysis of molecular mechanism of anti-asthmatic activity of allosamidin by focusing the function of chitinase-like proteins
| Project/Area Number |
23380062
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SHOHEI Sakuda 東京大学, 農学生命科学研究科, 准教授 (80192087)
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| Co-Investigator(Renkei-kenkyūsha) |
INOUE Hiromasa 鹿児島大学, 大学院・医歯学総合研究科, 教授 (30264039)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 生物活性物質 / 喘息 / キチナーゼ / キチナーゼ様タンパク質 / アロサミジン |
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| Research Abstract |
Allosamidin, a chitinase inhibitor, shows anti-asthmatic activity toward model mice. Chitinase (AMCase) and chitinase-like proteins (BRP39, Ym1, and Ym2) are possible candidates as target molecules for anti-asthmatic activity of allosamidin. To Recombinant protein of each of the four proteins was prepared and their binding activity toward allosamidin probes, which were newly prepared, was investigated. All the recombinant proteins bound to allosamidin with low Kd values around 10-100 nM. It was found that BRP39 shows protecting activity against death of mouse primary cells. Allosamidin did not inhibit the activity of BRP39 for the cells.
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[Journal Article]2011
Author(s)
Yosuke Sato, Shigeo Suzuki, Seiko Muraoka, Naoya Kikuchi, Naotaka Noda, Takafumi Matsumoto, Hiromasa Inoue, Hiromichi Nagasawa, and Shohei Sakuda
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Journal Title
Bioorg. Med. Chem
Volume: 19
Pages: 3054-3059
DOI
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