2013 Fiscal Year Final Research Report

Studies on biliary and non-biliary pathways of cholesterol excretion and its regulation by food factors

Research Project

Project/Area Number	23380071
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	一般
Research Field	Food science
Research Institution	Tohoku University
Principal Investigator	IKEDA Ikuo 東北大学, (連合)農学研究科(研究院), 教授 (40136544)
Project Period (FY)	2011-04-01 – 2014-03-31
Keywords	コレステロール / 胆汁 / 非胆汁 / 小腸内腔 / コレステロール排泄 / ABCG5/ABCG8 / 胆汁酸
Research Abstract	We tried to establish an experimental method to compare biliary and non-biliary excretion of cholesterol in rats. Cholesterol excretion through bile in overnight fasting rats was about two times higher than that in the feeding rats. Cholesterol excretion through non-biliary pathway was lower in the fasting rats compared with the feeding rats. The administration of LXR agonist increased non-biliary cholesterol excretion, but not biliary cholesterol excretion. Both biliary and non-biliary cholesterol excretion were increased in the feeding of a high cholesterol diet. These changes in biliary and non-biliary cholesterol excretion could not be necessarily explained by the mRNA expression of ABCG5 and ABCG8. Therefore, more studies are necessary to establish mechanisms of the regulation of cholesterol excretion through biliary and non-biliary pathways.