2013 Fiscal Year Final Research Report
Nutrient sensing and signaling mechanisms of hepatic and intestinal autophagy
| Project/Area Number |
23380074
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Food science
|
|---|
| Research Institution | Niigata University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
FUJIMURA Shinobu 新潟大学, 自然科学系, 准教授 (20282999)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
KATO Hisanori 東京大学, 総括プロジェクト機構, 特任教授 (40211164)
|
|---|
| Project Period (FY) |
2011-04-01 – 2014-03-31
|
| Keywords | オートファジー / アミノ酸 / 肝細胞 / LC3 / アルギニン / 活性酸素種 / サーチュイン |
|---|
| Research Abstract |
Autophagy is a ubiquitous, intracellular system to degrade and recycle cellular components. The nutritional regulation of autophagy was investigated in rat hepatoma H4-II-E cells and gastrointestinal tracts. Especially, the mechanism of regulation, various signaling mechanisms of each amino acid was proved to be ROS-dependent and -independent. Arginine uses NO pathway and mTOR-independent. The level of mRNA of LC3 molecule, an autophagy marker, and related Atg genes regulated by amino acids was discovered. Anti-oxidative food components, epigallocathecin gallate and resveratrol, stimulated autophagy. Finally, the relationship of amino acid regulation of autophagy with sirtuins, an anti-aging gene, were investigated.
|
Research Products
(31 results)
