2013 Fiscal Year Final Research Report
Laser microdissection-assisted analysis of ligninication and related gene expression in the microstructure of woody lignifiying cells
| Project/Area Number |
23380102
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Wood science
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| Research Institution | Kyushu University |
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Principal Investigator |
TSUTSUMI YUJI 九州大学, (連合)農学研究科(研究院), 教授 (30236921)
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| Co-Investigator(Kenkyū-buntansha) |
FUJITA Koki 九州大学, 大学院・農学研究院, 助教 (90264100)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | リグニン / 生合成 / 遺伝子 / 発現解析 / 微細組織 / レーザーマイクロダイセクション / Py-GC/MS |
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| Research Abstract |
The cells were collected from the different stages of lignification or different organs by a laser micro-dissection (LMD) technique and were subjected to the analyses of lignin structure and gene expression.
Transcriptions of the lignification related genes were analyzed by Real-time PCR. COMT, CCoAOMT, and CAD, were transcribed prior to lignin deposition. The transcription levels of CCR and 4CL were lower than them. CCR stands in the lignification specific pathway; therefore it is considered to be a regulatory gene in the lignin biosynthesis. The lignin structure analysis was achieved with only 0.1 square millimeter samples as a minimum requirement by the combined LMD and Py-GC/MS. The results indicated that syringyl lignin is synthesized in the beginning of lignification, and that structural changes of lignification still proceeds even in the area in which lignification seems already completed by the histochemical observations.
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