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2013 Fiscal Year Final Research Report

Laser microdissection-assisted analysis of ligninication and related gene expression in the microstructure of woody lignifiying cells

Research Project

  • PDF
Project/Area Number 23380102
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Wood science
Research InstitutionKyushu University

Principal Investigator

TSUTSUMI YUJI  九州大学, (連合)農学研究科(研究院), 教授 (30236921)

Co-Investigator(Kenkyū-buntansha) FUJITA Koki  九州大学, 大学院・農学研究院, 助教 (90264100)
Project Period (FY) 2011-04-01 – 2014-03-31
Keywordsリグニン / 生合成 / 遺伝子 / 発現解析 / 微細組織 / レーザーマイクロダイセクション / Py-GC/MS
Research Abstract

The cells were collected from the different stages of lignification or different organs by a laser micro-dissection (LMD) technique and were subjected to the analyses of lignin structure and gene expression.
Transcriptions of the lignification related genes were analyzed by Real-time PCR. COMT, CCoAOMT, and CAD, were transcribed prior to lignin deposition. The transcription levels of CCR and 4CL were lower than them. CCR stands in the lignification specific pathway; therefore it is considered to be a regulatory gene in the lignin biosynthesis. The lignin structure analysis was achieved with only 0.1 square millimeter samples as a minimum requirement by the combined LMD and Py-GC/MS. The results indicated that syringyl lignin is synthesized in the beginning of lignification, and that structural changes of lignification still proceeds even in the area in which lignification seems already completed by the histochemical observations.

  • Research Products

    (7 results)

All 2014 2013 2012

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (6 results) (of which Invited: 1 results)

  • [Journal Article] Putative Cationic Cell-Wall-Bound Peroxidase Homologues in Arabidopsis, AtPrx2, AtPrx25, and AtPrx71, Are Involved in Lignification2013

    • Author(s)
      Jun Shigeto, Koki Fujita, Yuko Kiyonaga, Ryuichiro Kondo, Yuji Tsutsumi
    • Journal Title

      J. Agric. Food Chem

      Volume: 61(16) Pages: 3781-3788

    • Peer Reviewed
  • [Presentation] リグニンの高分子化を担う植物ペルオキシダーゼの酸化能と酸化機構2014

    • Author(s)
      重藤 潤, 堤 祐司
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学
    • Year and Date
      2014-03-20
  • [Presentation] CWPO-C の転写およびプロモーター解析2014

    • Author(s)
      大平 香織, Kasturi Banerjee, 重藤 潤, 堤 祐司
    • Organizer
      第64回日本木材学会大会
    • Place of Presentation
      愛媛大学
    • Year and Date
      2014-03-14
  • [Presentation] リグニンを高分子化する植物ペルオキシダーゼの酸化能2013

    • Author(s)
      重藤 潤, 堤 祐司
    • Organizer
      第58回リグニン討論会
    • Place of Presentation
      香川大学
    • Year and Date
      2013-11-12
  • [Presentation] リグニン高分子化ペルオ キシダーゼの反応特性2013

    • Author(s)
      堤 祐司, 重藤 潤, 長野 万里子
    • Organizer
      第77回日本植物学会大会
    • Place of Presentation
      北海道大学
    • Year and Date
      2013-09-13
  • [Presentation] Laser microdissection を用いたリグニン不均一性制御機構の解明2013

    • Author(s)
      和田 卓, 藤田 弘毅, 堤 祐司
    • Organizer
      第63回日本木材学会大会
    • Place of Presentation
      岩手大学
    • Year and Date
      2013-03-27
  • [Presentation] マイクロダイセクション : 微細組織におけるリグニン構造と生合成遺伝子の発現2012

    • Author(s)
      堤 祐司
    • Organizer
      組織と材質研究会2012年度秋期シンポジウム
    • Place of Presentation
      東京農工大学府中キャンパス
    • Year and Date
      2012-09-26
    • Invited

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Published: 2015-06-25  

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