2013 Fiscal Year Final Research Report
Elucidation of mechanism of neuroendocrine control in reproduction for advancement of artificial seed production of commercial bivalve mollusks
| Project/Area Number |
23380109
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | Tohoku University |
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Principal Investigator |
MAKOTO Osada 東北大学, (連合)農学研究科(研究院), 教授 (30177208)
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| Co-Investigator(Kenkyū-buntansha) |
ITOH Naoki 東北大学, 大学院・農学研究科, 助教 (30502736)
CHIBA Hiroaki 北里大学, 水産学部, 准教授 (50236816)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 二枚貝 / 神経内分泌 / エストロゲンシグナリング / 配偶子形成 / 産卵 |
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| Research Abstract |
We cloned the cDNA transcripts of prepro Gonadotropin Releasing Hormones (GnRH)-like peptides from the central nervous system of scallop(pyGnRH)and oyster (cgGnRH). The putative pyGnRH peptide stimulated the proliferation of spermatogonia, which was mediated through the induction of estrogen synthesis by pyGnRH.
We successfully cloned two novel forms of estrogen receptor from two kinds of mussels and Japanese scallop. These receptors were classified each into an estrogen receptor (ER) and estrogen-related receptor (ERR) and were expressed in both ovary and testis. The flounder ERa-mediated gene assay suggested that pyER behaves as a transcription factor to estrogen while pyERR is a constitutive activator.
Serotonin (5-HT)-induced oocyte maturation and sperm motility is inhibited by oocyte maturation arresting factor (OMAF) due to interference of extracellular Ca2+ influx. The release from suppressive OMAF due to absorption with antibody against OMAF amplified the 5-HT-induced spawning.
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[Journal Article] Mollusc gonadotropin-releasing hormone directly regulates gonadal functions : A primitive endocrine system controlling reproduction2012
Author(s)
Nicholas Treen, Naoki Itoh, Hanae Miura, Ippei Kikuchi, Takenori Ueda, Keisuke G. Takahashi, Takayoshi Ubuka, Kazutoshi Yamamoto, Peter J. Sharp, Kazuyoshi Tsutsui, and Makoto Osada
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Journal Title
Gen. Comp. Endocrinol
Volume: 176
Pages: 167-172
DOI
Peer Reviewed
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[Presentation] Mollusc gonadotropin-releasing hormone directly regulates gonadal functions : a primitive endocrine system controlling reproduction2012
Author(s)
Treen N., Itoh N., Miura H., Kikuchi I., Ueda T., Takahashi K.G., Ubuka T., Yamamoto K., Sharp P.J., Tsutsui K., Osada M
Organizer
The Seventh Congress of the Asia and Oceania Society for Comparative Endocrinology (AOSCE)
Place of Presentation
Kuala Lumpur, Malaysia
Year and Date
20120303-07
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[Presentation] Bivalve Mollusc Gonadotropin-Releasing Hormone2011
Author(s)
Nicholas Treen, Naoki Itoh, Hanae Miura, Ippei Kikuchi, Takenori Ueda, Keisuke G.Takahashi , Takayoshi Ubuka , Kazutoshi Yamamoto , Kazuyoshi Tsutsui , Peter Sharp , Makoto Osada
Organizer
第36回日本比較内分泌学会大会
Place of Presentation
東京都千代田区
Year and Date
20111122-25
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