2013 Fiscal Year Final Research Report
Development of biosensor systems for fish health screening
| Project/Area Number |
23380122
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Fisheries chemistry
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| Research Institution | Tokyo University of Marine Science and Technology |
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Principal Investigator |
ENDO Hideaki 東京海洋大学, 海洋科学技術研究科, 教授 (50242326)
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| Co-Investigator(Kenkyū-buntansha) |
OHNUKI Hitoshi 東京海洋大学, 大学院・海洋工学系, 准教授 (60223898)
YOSHIURA Yasutoshi 独立行政法人水産総合研究センター, 瀬戸内海区水産研究所, 主任研究員 (90372052)
MURATA Masataka 函館地域産業振興財団, 北海道立工業技術センター, 研究主任 (40505707)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | バイオセンサ / 魚類 / 健康診断 / モニタリング / リアルタイム |
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| Research Abstract |
We developed novel biosensor systems for fish health screening. Various implantable wireless-biosensors to monitor fish health indicators, such as glucose, cholesterol, and lactic acid, were prepared. These sensor systems allow us to monitor the health indicators in fish under free-swimming conditions. We also developed a label-free immunosensor system for detecting the oocyte maturation-inducing hormone (DHP). The mechanism of the sensor system is based on differences in electrochemical activity changed by an immunoreaction in the absence and presence of DHP. One sample can be used to measure DHP levels within as little as 15 min. Moreover, we developed a novel method for detecting Flavobacterium psychrophilum using high-gradient immunomagnetic separation with polymerase chain reaction, which allows for ultra-highly sensitive and specific detection of F. psychrophilum. These newly developed biosensor systems allow for rapid and convenient monitoring of fish health indicators.
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