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2013 Fiscal Year Final Research Report

Development of biosensor systems for fish health screening

Research Project

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Project/Area Number 23380122
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Fisheries chemistry
Research InstitutionTokyo University of Marine Science and Technology

Principal Investigator

ENDO Hideaki  東京海洋大学, 海洋科学技術研究科, 教授 (50242326)

Co-Investigator(Kenkyū-buntansha) OHNUKI Hitoshi  東京海洋大学, 大学院・海洋工学系, 准教授 (60223898)
YOSHIURA Yasutoshi  独立行政法人水産総合研究センター, 瀬戸内海区水産研究所, 主任研究員 (90372052)
MURATA Masataka  函館地域産業振興財団, 北海道立工業技術センター, 研究主任 (40505707)
Project Period (FY) 2011-04-01 – 2014-03-31
Keywordsバイオセンサ / 魚類 / 健康診断 / モニタリング / リアルタイム
Research Abstract

We developed novel biosensor systems for fish health screening. Various implantable wireless-biosensors to monitor fish health indicators, such as glucose, cholesterol, and lactic acid, were prepared. These sensor systems allow us to monitor the health indicators in fish under free-swimming conditions. We also developed a label-free immunosensor system for detecting the oocyte maturation-inducing hormone (DHP). The mechanism of the sensor system is based on differences in electrochemical activity changed by an immunoreaction in the absence and presence of DHP. One sample can be used to measure DHP levels within as little as 15 min. Moreover, we developed a novel method for detecting Flavobacterium psychrophilum using high-gradient immunomagnetic separation with polymerase chain reaction, which allows for ultra-highly sensitive and specific detection of F. psychrophilum. These newly developed biosensor systems allow for rapid and convenient monitoring of fish health indicators.

  • Research Products

    (28 results)

All 2014 2013 2012 2011 Other

All Journal Article (12 results) (of which Peer Reviewed: 11 results) Presentation (13 results) Book (1 results) Remarks (2 results)

  • [Journal Article] Development of mediator-type biosensor to wirelessly monitor whole cholesterol concentration in fish2014

    • Author(s)
      M. Takase, M. Murata, K. Hibi, R. Huifeng, H. Endo
    • Journal Title

      Fish Physiol. Biochem

      Volume: 40 Pages: 385-394

    • Peer Reviewed
  • [Journal Article] 魚類の健康状態のリアルタイム診断の可能性~バイオセンサを用いた「さかなドック」の創出~2014

    • Author(s)
      遠藤英明
    • Journal Title

      科学・技術研究

      Volume: 2 Pages: 85-90

  • [Journal Article] Carbon nanotube enhanced label-free immunosensor for amperometric determination of oocyte maturation-inducing hormone in fish2013

    • Author(s)
      M. Hirai, T. Muramatsu, H. Ohnuki, K. Hibi, H. Ren, H. Endo
    • Journal Title

      Fish Physiol. Biochem

      Volume: 39 Pages: 299-308

    • Peer Reviewed
  • [Journal Article] Development of a biocompatible glucose biosensor for wireless and real time blood glucose monitoring of fish2013

    • Author(s)
      M. Takase, E. Takahashi, M. Murata, H. Ohnuki, K. Hibi, H. Ren, H. Endo
    • Journal Title

      Int. J. Environ. Anal. Chem

      Volume: 93 Pages: 125-139

    • Peer Reviewed
  • [Journal Article] Development of a label-free immunosensor system for detecting oocyte maturation- inducing hormone in fish2012

    • Author(s)
      H. Endo, T. Muramatsu, G. Yoshizaki, H. Ren, H. Ohnuki
    • Journal Title

      Fish. Sci

      Volume: 78 Pages: 391-398

    • Peer Reviewed
  • [Journal Article] Ultra highly sensitive method for detecting Flavobacterium psychrophilum using high-gradient immunomagnetic separation with polymerase chain reaction2012

    • Author(s)
      U. Ryumae, K. Hibi, Y. Yoshiura, H. Ren, H. Endo
    • Journal Title

      Aquaculture Res

      Volume: 43 Pages: 929-939

    • Peer Reviewed
  • [Journal Article] Carbon nanotube enhanced mediator-type biosensor for real-time monitoring of glucose concentrations in fish2012

    • Author(s)
      M. Takase, Y. Yoneyama, M. Murata, K. Hibi, H. Ren, H. Endo
    • Journal Title

      Anal. Bioanal. Chem

      Volume: 403 Pages: 1187-1190

    • Peer Reviewed
  • [Journal Article] Mediator-type biosensor for real-time wireless monitoring of blood glucose concentrations in fish2012

    • Author(s)
      M. Takase, Y. Yoneyama, M. Murata, K. Hibi, H. Ren, H. Endo
    • Journal Title

      Fish. Sci

      Volume: 78 Pages: 691-698

    • Peer Reviewed
  • [Journal Article] Rapid detection of Flavobacterium psychrophilum with magneticfluorescent beads and flow cytometry2012

    • Author(s)
      K. Hibi, Y. Yoshiura, H. Endo
    • Journal Title

      Sens. Material

      Volume: 24 Pages: 311-322

    • Peer Reviewed
  • [Journal Article] Wireless biosensor system for real-time L-lactic acid monitoring in fish2012

    • Author(s)
      K. Hibi, K. Hatanaka, M. Takase, H. Ren, H. Endo
    • Journal Title

      Sensors

      Volume: 12 Pages: 6269-6281

    • Peer Reviewed
  • [Journal Article] Electrode-based immunologic assay system to monitor oocyte maturation-inducing hormone in fish2011

    • Author(s)
      H. Endo, M. Igarashi, A. Banba, H. Ohnuki, H. Ushio, T. Hayashi, H. Ren, G. Yoshizaki
    • Journal Title

      Int. J. Environ. Anal. Chem

      Volume: 91 Pages: 174-184

    • Peer Reviewed
  • [Journal Article] Electrochemical flow injection immunoassay for cortisol using magnetic microbeads2011

    • Author(s)
      T. Muramatsu, H. Ohnuki, H. Ushio, K. Hibi, M. Igarashi, T. Hayashi, H. Ren, H. Endo
    • Journal Title

      Int. J. Environ. Anal. Chem

      Volume: 91 Pages: 161-173

    • Peer Reviewed
  • [Presentation] カーボンナノチューブを用いた魚類のためのコルチゾル測定用 : イムノセンサの改良に関する研究2014

    • Author(s)
      呉海雲, 大貫等, 日比香子, 任恵峰, 遠藤英明
    • Organizer
      日本水産学会春季大会
    • Place of Presentation
      北海道大学
    • Year and Date
      2014-03-29
  • [Presentation] Development of label-free biosensor for detecting steroid hormone concentration in fish2013

    • Author(s)
      H. Wu, H. Endo
    • Organizer
      International Symposium on Aquatic Products Processing
    • Place of Presentation
      Indonesia
    • Year and Date
      20131111-16
  • [Presentation] Development of a label-free immunosensor system for detecting plasma cortisol levels in fish2013

    • Author(s)
      H. Wu, K. Hibi, H. Ohnuki, H. Ren, H. Endo
    • Organizer
      aquaculture 2013
    • Place of Presentation
      Gran Canaria, Spain
    • Year and Date
      20131103-06
  • [Presentation] カーボンナノチューブ(CNT)を用いた魚類のためのコレステロールバイオセンサの開発に関する研究2013

    • Author(s)
      陶格斯, 村田政隆, 高瀬麻以, 日比香子, 任恵峰, 遠藤英明
    • Organizer
      日本水産学会秋季大会
    • Place of Presentation
      三重大学
    • Year and Date
      2013-09-20
  • [Presentation] 固定化抗体磁性ビーズを用いたフローサイトメトリーによるEdwardsiella ictaluri の検出2013

    • Author(s)
      日比香子, 平井睦子(海洋大), 吉浦康寿(水研セ増養殖研), 任 恵峰, 遠藤英明(海洋大)
    • Organizer
      日本水産学会春季大会
    • Place of Presentation
      東京海洋大学
    • Year and Date
      2013-03-27
  • [Presentation] Simultaneous real-time monitoring of glucose and cholesterol levels in fish using anenzyme-based sensor2012

    • Author(s)
      K. Hibi, M. Murata, T. Oomiri, M. Takase, H. Ren, H. Endo
    • Organizer
      Biosensors 2012
    • Place of Presentation
      Cancún, Mexico
    • Year and Date
      20120515-18
  • [Presentation] Implantable mediator-type biosensor for continuous in vivo monitoring of total cholesterol concentration in fish2012

    • Author(s)
      M. Takase, M. Murata, K. Hibi, H. Ren, H. Endo
    • Organizer
      Biosensors 2012
    • Place of Presentation
      Cancún, Mexico
    • Year and Date
      20120515-18
  • [Presentation] 魚類のストレス状態測定のためのメディエータ型バイオセンサの試作2012

    • Author(s)
      高瀬麻以, 村田政隆, 日比香子, 任 恵峰, 遠藤英明
    • Organizer
      日本水産学会秋季大会
    • Place of Presentation
      下関水産大学校
    • Year and Date
      2012-09-15
  • [Presentation] アユ冷水病菌検出のための非標識免疫センサの試作2012

    • Author(s)
      平井睦子, 日比香子, 吉浦康寿, 任恵峰, 遠藤英明
    • Organizer
      日本水産学会春季大会
    • Place of Presentation
      東京海洋大学
    • Year and Date
      2012-03-28
  • [Presentation] Wireless biosensor system for real-time L-lactic acid monitoring in fish2011

    • Author(s)
      K. Hibi, K. Hatanaka, M. Takase, H. Ren, H. Endo
    • Organizer
      Euroanalysis 2011
    • Place of Presentation
      Belgrade, Serbia
    • Year and Date
      20110911-15
  • [Presentation] Label-free immunosensor using carbon nanotube for amperometric determination of oocyte maturation-inducing hormone in fish2011

    • Author(s)
      M. Hirai, T. Muramatsu, H. Ohnuki, G. Yoshizaki, K. Hibi, H. Ren, H. Endo
    • Organizer
      Euroanalysis 2011
    • Place of Presentation
      Belgrade, Serbia
    • Year and Date
      20110911-15
  • [Presentation] Development of carbon nanotube modified mediator biosensor for real time monitoring of blood glucose levels in fish2011

    • Author(s)
      M. Takase, Y. Yoneyama, M. Murata, K. Hibi, H. Ren, H. Endo
    • Organizer
      Euroanalysis 2011
    • Place of Presentation
      Belgrade, Serbia
    • Year and Date
      20110911-15
  • [Presentation] Development of mediator type biosensor for continuous measurement of blood glucose levels in fish2011

    • Author(s)
      M. Takase, Y. Yoneyama, M. Murata, H. Ren, H. Endo
    • Organizer
      The 10th workshop on biosensors and bioanalytical microtechniques in environmental and clinical analysis (BBMEC 2011)
    • Place of Presentation
      Weimar, Germany
    • Year and Date
      20110619-22
  • [Book] バイオセンサの先端科学技術と新製品への応用開発, 第9章第9節「バイオセンサによる迅速・簡便な魚類の健康診断~さかなドックの創出に向けて~」2014

    • Author(s)
      遠藤英明(分担執筆)
    • Total Pages
      550(377-382)
    • Publisher
      技術情報協会, 東京
  • [Remarks] ■遠藤英明研究室

    • URL

      http://www2.kaiyodai.ac.jp/~endo/endo/index.html

  • [Remarks] ■東京海洋大学研究者総覧データベース

    • URL

      http://olcr.kaiyodai.ac.jp/kenkyusha-db/kaiyokagaku.html

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Published: 2015-06-25  

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