2013 Fiscal Year Final Research Report
Modification of the fatty acid biosynthetic pathway by transgenesis in a marine teleost
| Project/Area Number |
23380123
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Fisheries chemistry
|
|---|
| Research Institution | Tokyo University of Marine Science and Technology |
|---|
Principal Investigator |
YOSHIZAKI Goro 東京海洋大学, 海洋科学技術研究科, 教授 (70281003)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SATOH Shuichi 東京海洋大学, 大学院・海洋科学技術研究科, 教授 (80154053)
HAGA Yutaka 東京海洋大学, 大学院・海洋科学技術研究科, 准教授 (00432063)
|
|---|
| Project Period (FY) |
2011-04-01 – 2014-03-31
|
| Keywords | 脂肪酸代謝酵素 / 海産魚 / 遺伝子導入 |
|---|
| Research Abstract |
Marine fishes are generally unable to produce sufficient eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). It is therefore necessary to supplement cultured marine fish species diets with fish oils to supply EPA and DHA. Given that freshwater fishes can synthesize both EPA and DHA, they presumably express all of the enzymes required for this pathway. Therefore, we used marine fish, nibe croaker to produce a transgenic line carrying the elongase gene isolated from masu salmon. Fatty acid analysis revealed that the liver EPA content in the transgenic fish was lower. However, docosapentaenoic acid content in the transgenic fish was 2.28-fold higher than in non-transgenic fish. Further, tetracosapentaenoic acid was specifically detected in the transgenic fish. We therefore conclude that the development of transgenic fish lines with these fatty acid-metabolizing enzymes could be a powerful tool for manipulating fatty acid metabolic pathways in fish.
|
