2013 Fiscal Year Final Research Report
A study on mechanism of tetrodotoxin poisoning in tiger pufferfish by binding protein
| Project/Area Number |
23380125
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Fisheries chemistry
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| Research Institution | Kyushu University |
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Principal Investigator |
OSHIMA Yuji 九州大学, (連合)農学研究科(研究院), 教授 (70176874)
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| Co-Investigator(Kenkyū-buntansha) |
KINOSHITA Masato 京都大学, 大学院・農学研究院, 助教 (60263125)
HASHIGUCHI Yasuyuki 大阪医科大学, 医学部, 助教 (70436517)
SHIMASAKI Yohei 九州大学, 大学院・農学研究院, 准教授 (40363329)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 天然毒 / フグ毒 / トリブチルスズ / 結合タンパク質 |
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| Research Abstract |
In this study, we performed to elucidate the mechanism of tetrodotoxin (TTX) poisoning in tiger pufferfish. First, recombinants of TTX binding protein and tributyltin (TBT) binding protein were successfully obtained from Bombyx mori. The results of binding assay using the recombinant proteins showed that these proteins could bind with TTX or TBT suggesting their detoxification function. In addition, transgenic medaka strains which over expressed TTX or TBT binding protein were successfully established. The results of TBT injection test to the transgenic medaka embryo suggested its detoxification function. Molecular phylogenic study of toxin binding protein among several puffer fish species demonstrated a evolution pathway from TBT binding protein to TTX binding protein.
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Research Products
(16 results)
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[Presentation] フグ毒結合タンパク質の魚種間分布2013
Author(s)
小松正治, 李 在萬, 橋口康之, 白石真, 野中翔平, 島崎洋平, 望岡典隆, 日下部宜宏, 大嶋雄治
Organizer
平成25年度日本水産学会春季大会
Place of Presentation
東京海洋大学
Year and Date
2013-03-27
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[Presentation] フグ毒結合タンパク質の分子進化2012
Author(s)
白石 真, 李 在萬, 橋口康之, 小松正治, 三木志津帆, 島崎洋平, 望岡典隆, 日下部宣宏, 大嶋雄治
Organizer
平成23年度水産学会九州支部大会
Place of Presentation
鹿児島市, 鹿児島大学
Year and Date
2012-01-28
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