2013 Fiscal Year Final Research Report
Analysis of trophoblastic cell lineages and mechanism for multinucleation in bovine trophoblastic cell lines
| Project/Area Number |
23380162
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Iwate University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KIZAKI Keiichiro 岩手大学, 農学部, 准教授 (40337994)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 細胞・組織 / 遺伝子 / ゲノム / マイクロアレイ / 発生・分化 |
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| Research Abstract |
Trophoblastic cells, derived from the outer layer of morula, are key cell for implantation and placentation. Bovine trophoblastic cells contain two specific cells; mononucleate and binucleate, and they express and produce various specific molecules, individually. In this study, we examined the mechanism of trophoblastic cell lineage, namely morphological and functional differentiation, using previously established cell lines. Matrigel, which derived from extra-cellular matrix, effectively induced trophoblastic cell differentiation. The bi-nucleation associated with increasing expression of retro-element. The differentiated cells produced retro-element molecule, fematrin-1, which was named by fetomaternal trinucleate cell inducer 1, and it, has a fusion activity between trophoblastic cells and endometrial cells in bovine. These results showed a direct evidence for cell-to-cell fusion in bovine placenta and open a new sight for bovine reproduction, especially early gestation.
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[Presentation] Bovine trophoblastic cell differentiation and binucleation involved enhanced endogenous retrovirus element expression2012
Author(s)
Koshi K, Suzuki Y, Nakaya Y, Imai K, Hosoe M, Takahashi T, Kizaki K, Miyazawa T, Hashizume K
Organizer
45th Annual Meeting Society for the Study of Reproduction
Place of Presentation
The Pennsylvania State University, State College, Pennsylvania, USA
Year and Date
2012-08-12
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[Presentation] Gene expression profiles and differentiation in new developing bovine trophoblastic cell line2012
Author(s)
Hashizume K, Koshi K, Suzuki Y, Kizaki K, Imai K, Ito A, Takahashi T, Hosoe M, Watanabe D
Organizer
17th International Congress on Animal Reproduction (ICAR)
Place of Presentation
Vancouver Convention Centre, Vancouver, Canada
Year and Date
2012-07-29
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[Presentation] Hashizume K, Koshi K, Ushizawa K, Nakaya Y, Takahashi T, Kizaki K, Hosoe M, Miyazawa T, Suzuki Y2011
Author(s)
Hashizume K, Koshi K, Ushizawa K, Nakaya Y, Takahashi T, Kizaki K, Hosoe M, Miyazawa T, Suzuki Y
Organizer
The 2nd World Congress on Reproductive Biology
Place of Presentation
Cairns Convention Center Cairns, Australia
Year and Date
2011-10-11
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