Epigenetic analysis of zygotic gene activation by maternal factor

2014 Fiscal Year Final Research Report

• Project/Area Number: 23380164
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied animal science
• Research Institution: Kyoto University
• Principal Investigator: MINAMI Naojiro 京都大学, (連合)農学研究科(研究院), 准教授 (30212236)
• Co-Investigator(Kenkyū-buntansha): MATSUMOTO Kazuya 近畿大学, 生物理工学部, 教授 (20298938) ; TSUKAMOTO Satoshi 放射線医学総合研究所, 主任研究員 (80510693)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Keywords: マウス初期胚 / エピジェネティクス / 胚性ゲノムの活性化 / 遺伝子発現 / 母性因子 / siRNA / 多能性 / 分化

Outline of Final Research Achievements

Smyd3 is a histone H3 lysine 4 (H3K4) di- and tri-methyltransferase that forms a transcriptional complex with RNA polymerase II and CHD1, which recognizes trimethylated histone H3 lysine 4, is a protein belonging to the family of ATPase-dependent chromatin remodeling factors. In the present study, we investigated the effects of RNA interference (RNAi)-mediated repression on the development of mouse embryos. In Smyd3-, and Chd1-knockdown embryos, the percentage of inner cell mass (ICM)-derived colony formation and trophectoderm (TE)-derived cell attachment was significantly decreased, resulting in a reduction in the number of viable offspring. Furthermore, the expression of Pou5f1, Nanog, and Cdx2, was dramatically decreased in both Smyd3- and Chd1-knockdown embryos. Moreover, in Chd1-knockdown embryos, expression of Hmgpi, which regulate Pou5f1, Nanog, and Cdx2, was also significantly suppressed at zygotic gene activation (ZGA).

Free Research Field

生殖生物学