2013 Fiscal Year Final Research Report
The improvement of the tolerance of mammalian oocytes to cryopreservation by inducing endogenous expression of water/cryoprotectant channels or by gating the channels
| Project/Area Number |
23380167
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Kochi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KASAI Magosaburo 高知大学, 教育研究部総合科学系, 教授 (60152617)
KOSHIMOTO Chihiro 宮崎大学, フロンティア科学実験総合センター, 教授 (70295210)
MATSUKAWA Kazutsugu 高知大学, 教育研究部総合科学系, 准教授 (00532160)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 繁殖 |
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| Research Abstract |
To increase the survival of mouse oocytes after vitrification, we first tried to increase the permeability of their inner mitochondrial membrane. When oocytes were treated with phenylarsine oxide to open the mitochondrial permeability transition pore, a non-specific channel present in the inner membrane, the survival of vitrified oocytes increased significantly just after warming. However, they were dead after 1 h of culture. Then, we tried to increase the expression of water channels, which are expressed in the inner membrane. However, the tolerance to vitrification was not improved, probably because the expression was not increased. Next, we cryopreserved oocytes by equilibrium vitrification to promote dehydration/concentration of the cell and mitochondria. The survival was high even if they were warmed in the air, suggesting that they were vitrified under a near equilibrium state. These results suggest that equilibrium vitrification is suitable for cryopreservation of mouse oocytes.
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Research Products
(36 results)
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[Journal Article] Increased metabolite levels of glycolysis and pentose phosphate pathway in rabbit atherosclerotic arteries and hypoxic macrophage2014
Author(s)
Atsushi Yamashita, Yan Zhao, Yunosuke Matsuura, Kazuaki Yamasaki, Sayaka Moriguchi-Goto, Chihiro Sugita, Takashi Iwakiri, Nozomi Okuyama, Chihiro Koshimoto, Keiichi Kawai, Nagara Tamaki, Songji Zhao, Yuji Kuge, Yujiro Asada
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Journal Title
PLos One
Volume: 9
Pages: e86426
DOI
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[Journal Article] Rapid movement of water and cryoprotectants in pig expanded blastocysts via channel processes : its relevance to their higher tolerance to cryopreservation2013
Author(s)
Bo Jin, Ryu-ichi Higashiyama, Yu-ichi Nakata, Jun-ichi Yonezawa, Shangdan Xu, Masashi Miyake, Sei-ichi Takahashi, Kazuhiro Kikuch, Ken-ichi Yazawa, Shuhei Mizobuchi, Saori Niimi, Mizuho Kitayama, Chihiro Koshimoto, Kazutsugu Matsukawa, Magosaburo Kasai, Keisuke Edashige
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Journal Title
Biology of Reproduction
Volume: 89
Pages: 1-12
DOI
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[Journal Article] Arterial 18F-fluorodeoxyglucose uptake reflects thrombus formation and tissue factor expression via nuclear factor-κB in rabbit atherosclerotic lesions2013
Author(s)
Atsushi Yamashita, Yan Zhao, Songji Zhao, Yunosuke Matsuura, Chihiro Sugita, Takashi Iwakiri, Nozomi Okuyama, Kazuyo Ohe, Chihiro Koshimoto, Keiichi Kawai, Nagara Tamaki, Yuji Kuge, Yujiro Asada
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Journal Title
Circulation Journal
Volume: 77
Pages: 2626-2635
DOI
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[Presentation] ^<18>F-FDG uptake reflects thrombus formation and tissue factor expression via nuclear factor-κB in rabbit atherosclerotic lesions2012
Author(s)
Atsushi Yamashita, Yan Zhao, Songji Zhao, Yunosuke Matsuura, Chihiro Sugita, Takashi Iwakiri, Kazuyo Ohe, Chihiro Koshimoto, Keiichi Kawai, Nagara Tamaki, Yuji Kuge, Yujiro Asada
Organizer
American Heart Association, Scientific Session
Place of Presentation
JW Marriott Los Angeles at LA Live, Los Angeles, California
Year and Date
20121103-07
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[Presentation] The role of aquaporin 9 in the movement of DMSO and acetamide in mouse morulae2012
Author(s)
Keisuke Edashige, Shinsuke Seki, Hayato Arimura, Kan Matsuo, Yu-ichi Nakata, Bo Jin, Kazutsugu Matsukawa, Chihiro Koshimoto, Magosaburo Kasai
Organizer
SSR's 45th Annual Meeting of Society of the Study of Reprocution
Place of Presentation
The Alumni Hall at the Hetzel Union Building, The Pennsylvania State University, University Park, Pennsylvania
Year and Date
20120812-15
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