2013 Fiscal Year Final Research Report
The improvement of the tolerance of mammalian oocytes to cryopreservation by inducing endogenous expression of water/cryoprotectant channels or by gating the channels
| Project/Area Number |
23380167
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Kochi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KASAI Magosaburo 高知大学, 教育研究部総合科学系, 教授 (60152617)
KOSHIMOTO Chihiro 宮崎大学, フロンティア科学実験総合センター, 教授 (70295210)
MATSUKAWA Kazutsugu 高知大学, 教育研究部総合科学系, 准教授 (00532160)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 繁殖 |
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| Research Abstract |
To increase the survival of mouse oocytes after vitrification, we first tried to increase the permeability of their inner mitochondrial membrane. When oocytes were treated with phenylarsine oxide to open the mitochondrial permeability transition pore, a non-specific channel present in the inner membrane, the survival of vitrified oocytes increased significantly just after warming. However, they were dead after 1 h of culture. Then, we tried to increase the expression of water channels, which are expressed in the inner membrane. However, the tolerance to vitrification was not improved, probably because the expression was not increased. Next, we cryopreserved oocytes by equilibrium vitrification to promote dehydration/concentration of the cell and mitochondria. The survival was high even if they were warmed in the air, suggesting that they were vitrified under a near equilibrium state. These results suggest that equilibrium vitrification is suitable for cryopreservation of mouse oocytes.
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Journal Title
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Pages: e86426
DOI
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Journal Title
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Volume: 77
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[Presentation] ^<18>F-FDG uptake reflects thrombus formation and tissue factor expression via nuclear factor-κB in rabbit atherosclerotic lesions2012
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