Clinical application of the minimal residual disease monitoring in canine lymphoma

2013 Fiscal Year Final Research Report

• Project/Area Number: 23380182
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Clinical veterinary science
• Research Institution: The University of Tokyo
• Principal Investigator: TSUJIMOTO Hajime 東京大学, 農学生命科学研究科, 教授 (60163804)
• Co-Investigator(Kenkyū-buntansha): OHNO Koichi 東京大学, 大学院・農学生命科学研究科, 准教授 (90294660) ; FUJINO Yasuhito 東京大学, 大学院・農学生命科学研究科, 助教 (70401180)
• Co-Investigator(Renkei-kenkyūsha): NISHIMURA Ryohei 東京大学, 大学院・農学生命科学研究科, 教授 (80172708) ; UCHIDA Kazuyuki 東京大学, 大学院・農学生命科学研究科, 准教授 (10223554)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: 動物 / 犬 / リンパ腫 / 微小残存病変

Research Abstract

Overall survival durations after standard chemotherapies have been reported as long as 1 year. In the present study, we assessed the efficacy of the chemotherapeutic protocols objectively by quantification of the minimal residual disease, MRD, after chemotherapy with real-time PCR. Through the results of MRD monitoring, we could propose several strategies to improve the chemotherapeutic protocols such as dose change of the drugs, requirement of the consolidation chemotherapy, and the necessity of the early re-induction chemotherapy during complete remission. The present studies were conceivably very helpful to improve the chemotherapeutic modalities for canine lymphoma as well as indicative of the usefulness of canine lymphoma as an animal model for human non-Hodgkin lymphoma.

Research Products

• [Journal Article] Prognostic significance of the expression levels of the p16, p15, and p14 genes in dogs with high-grade lymphoma (2014)
  • Author(s): Fujiwara-Igarashi, A., Goto-Koshino, Y., Sato, M., Maeda, S., Igarashi, H., Takahashi, M., Fujino, Y., Ohno, K. and Tsujimoto, H
  • Journal Title: Vet. J Volume: 199 Pages: 236-244
  • DOI: 10.1016/j.tvjl.2013.11.004
• [Journal Article] Establishment of five canine lymphoma cell lines and tumor formation in a xenotransplantation model (2013)
  • Author(s): Umeki, S., Ema, Y., Suzuki, R., Kubo, M., Hayashi, T., Okamura, Y., Yamazaki, J., Tsujimoto, H., Tani, K., Hiraoka, H., Okuda, M. and Mizuno, T
  • Journal Title: J. Vet Med. Sci Volume: 75 Pages: 467-474
  • DOI: 10.1292/jvms.12-0448
• [Journal Article] The prognostic significance of minimal residual disease in the early phases of chemotherapy in dogs with high-grade B-cell lymphoma (2013)
  • Author(s): Sato, M., Yamazaki, J., Goto-Koshino, Y., Takahashi, M., Fujino, Y., Ohno, K. and Tsujimoto, H
  • Journal Title: Vet. J Volume: 195 Pages: 319-324
  • DOI: 10.1016/j.tvjl.2012.07.013
• [Journal Article] Evaluation of prognostic significance of BCL6 gene expression in canine high-grade B-cell lymphoma (2012)
  • Author(s): Sato, M., Goto-Koshino, Y., Kanemoto, H., Mochizuki, H., Takahashi, M., Fujino, Y., Ohno, K. and Tsujimoto, H
  • Journal Title: Vet. J Volume: 191 Pages: 108-114
  • DOI: 10.1016/j.tvjl.2010.12.006
• [Journal Article] Increase in minimal residual disease (MRD) in peripheral blood before clinical relapse in dogs with lymphoma that achieved complete remission after chemotherapy (2011)
  • Author(s): Sato, M., Yamazaki, J., Goto-Koshino, Y., Takahashi, M., Fujino, Y., Ohno, K. and Tsujimoto, H
  • Journal Title: J. Vet. Intern. Med Volume: 25 Pages: 292-296
  • DOI: 10.1111/j.1939-1676.2010.0675.x
• [Journal Article] Evaluation of of cytoreductive efficacy of vincristine, cyclophosphamide, and doxorubicin in dogs with lymphoma by measuring the number of neoplastic lymphoid cells with real-time polymerase chain reaction (2011)
  • Author(s): Sato, M., Yamazaki, J., Goto-Koshino, Y., Takahashi, M., Fujino, Y., Ohno, K. and Tsujimoto, H
  • Journal Title: J. Vet. Intern. Med Volume: 25 Pages: 285-291
  • DOI: 10.1111/j.1939-1676.2011.0686.x
• [Presentation] 犬の辺縁帯リンパ腫(marginal zone lymphoma)9頭における臨床的・臨床病理学的特徴 (2014)
  • Author(s): 水田賢司, 高橋雅, 藤野泰人, 日吉沙綾, 福島建次郎, 金本英之, 大野耕一, 坪井誠也, 内田和幸, 辻本元
  • Organizer: 第10回日本獣医がん学会
  • Place of Presentation: 大阪
  • Year and Date: 2014-01-25
• [Presentation] GeneScanning analysis of antigen receptor gene rearrangement in the intestinal endoscopeic biopsy specimens and its application to the diagnosis of small cell lymphoma in dogs (2013)
  • Author(s): Hiyoshi, S., Uchida, K., Nakashima, K., Fukushima, K., Kanemoto, H., Goto, Y., Fujino, Y., Ohno, K. and Tsujimoto, H
  • Organizer: The Asian Meeting of Animal Medicine Specialties (AMAMS) 2013
  • Place of Presentation: ボゴール, インドネシア
  • Year and Date: 2013-12-14
• [Presentation] 犬のリンパ腫におけるGeneScanningを用いた微小残存病変検出に関する研究 (2013)
  • Author(s): 日吉沙綾, 後藤裕子, 佐藤雅彦, 高橋雅, 藤野泰人, 大野耕一, 辻本元
  • Organizer: 第9回日本獣医内科学アカデミー学術大会
  • Place of Presentation: 横浜
  • Year and Date: 2013-02-23
• [Presentation] 犬のT-zoneリンパ腫16例における臨床的・臨床病理学的特徴 (2013)
  • Author(s): 水谷格之, 後藤裕子, 内田和幸, 高橋雅, 藤野泰人, 大野耕一, 辻本元
  • Organizer: 第9回日本獣医内科学アカデミー学術大会
  • Place of Presentation: 横浜
  • Year and Date: 2013-02-23
• [Presentation] 医学から学ぶイヌのリンパ腫治療 (2012)
  • Author(s): 辻本元
  • Organizer: 日本獣医内科学アカデミー/日本獣医臨床病理学会2011年大会
  • Place of Presentation: 横浜
  • Year and Date: 2012-02-18
  • Invited
• [Presentation] 特徴的な細胞形態を示す新しいタイプの犬のリンパ腫 -Mott cell リンパ腫- (2011)
  • Author(s): 辻本元
  • Organizer: 第13回日本臨床獣医学フォーラム年次大会
  • Place of Presentation: 東京
  • Year and Date: 2011-09-17
  • Invited