Development of evaluation method of soil microorganisms evenness

2013 Fiscal Year Final Research Report

• Project/Area Number: 23380196
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Boundary agriculture
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: SHINANO Takuro 独立行政法人農業・食品産業技術総合研究機構, 東北農業研究センター農業放射線研究センター, センター長 (20235542)
• Co-Investigator(Kenkyū-buntansha): OKAZAKI Keiki 独立行政法人農業・食品産業技術総合研究機構, 北海道農業研究センター・生産環境研究領域, 主任研究員 (40414750) ; SEKIGUCHI Hiroyuki 独立行政法人農業・食品産業技術総合研究機構, 近畿中国四国農業研究センター・水田作研究領域, 主任研究員 (50466009) ; IWATA Yukiyoshi 独立行政法人農業・食品産業技術総合研究機構, 農村工学研究所・農地基盤工学研究領域, 主任研究員 (70370591)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: 土壌微生物 / 一酸化二窒素 / メタゲノム / メタトランスクリプトーム / 凍結融解

Research Abstract

We focus on nitrous oxide emission during freeze and thaw process in the field, and to investigate the diversity and evenness of the soil microorganism, metagenomic and metatranscriptomic approaches were applied on DNA and mRNA levels. The results indicate that during the bio-geochemical process, it is rather evenness play an important role than diversity of species number, indicating that few number of species seems to be increased with the process. We have applied the Functional Single Cell separation technique and obtain several isolates which seem to grow and lead the process dominantly under the cold condition.

Research Products

• [Journal Article] Metagenomic analysis of the rhizosphere soil microbiome with respect to phytic acid utilization (2013)
  • Author(s): Unno, Y., Shinano, T
  • Journal Title: Microbes and Environments Volume: 28 Pages: 120-127
  • DOI: 10.1264/jsme2.ME12181
  • Peer Reviewed
• [Journal Article] Water and nitrate movements in an agricultural field with different soil frost depths : field experiments and numerical simulation (2013)
  • Author(s): Iwata, Y., Yazaki, T., Suzuki, S., Hirota, T
  • Journal Title: Annals of Glaciology Volume: 54 Pages: 157-165
  • DOI: 10.3189/2013AoG62A204
  • Peer Reviewed
• [Presentation] Decreasing soil frost depth and its influence on the soil water and nitrate movements in Tokachi, Hokkaido, Japan (2012)
  • Author(s): Iwata, Y., Hirota, T., Hayashi, M., Yazaki, T., Nemoto, M., Ohkubo, S., Yanai, Y., Suzuki, S
  • Organizer: Plant and Microbe Adaptations to Cold
  • Place of Presentation: 札幌, 日本
  • Year and Date: 20120612-14
  • Invited
• [Presentation] Metagenomic analysis on the rhizosphere soil microbiome (2011)
  • Author(s): Shinano, T
  • Organizer: 3rd International Workshop "Advances in Science and Technology of Natural Resources"
  • Place of Presentation: プコン, チリ
  • Year and Date: 20111102-04
  • Invited
• [Book] Metagenomic analysis on the rhizosphere soil microbial community. In : Frans J. de Brjin (ed.) Molecular Microbial Ecology of the Rhizosphere (2013)
  • Author(s): Unno, Y. and Shinano, T
  • Total Pages: 1269(1099-1103)
  • Publisher: Wiley-Blackwell Publishers
• [Book] Plant and microbe adaptations to cold in a changing world, p41-52, Possible change of water and nitrate cycles associated with the frost-depth decrease under climate change (2013)
  • Author(s): Iwata, Y., Hirota, T., Hayashi, M, Arima, J., Suzuki, S., Nemoto, M
  • Total Pages: 352
  • Publisher: Springer