2013 Fiscal Year Final Research Report
Molecular basis of protein cross-linking reactions for cellular functions
| Project/Area Number |
23380200
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
小嶋 聡一 独立行政法人理化学研究所, ライフサイエンス技術基盤研究センター (10202061)
秋山 真志 名古屋大学, 大学院・医学系研究科 (60222551)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 酵素 / 蛋白質架橋 / 細胞機能 |
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| Research Abstract |
Transglutaminases are the enzymes that catalyze protein cross-linking or modification via peptide-bound glutamine residues. These reactions are essential for the multiple biological processes such as blood coagulation, skin epidermis formation and apoptosis. In order to elucidate the physiological roles and expression sites on mammals, using highly reactive substrate peptides (obtained by us for these years), we analyzed expression pattern and established identification system of possible substrates. In the studies, expressions for RNA, protein (using antibody), in situ enzymatic activity were investigated. Furthermore, establishment of FRET system to detect intracellular activity was attempted. As a result, in vitro system was successful. In addition, Medaka (Oryzias latepis) as a model animal, was analyzed for the orthologues of transglutaminases: biochemical data and establishment of a couple of genetic loss fish was produced.
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