2013 Fiscal Year Final Research Report
Development of nano-sized DDS for nucleic acid drugs based on polypod-like structured nucleic acids
| Project/Area Number |
23390010
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Kyoto University |
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Principal Investigator |
NISHIKAWA Makiya 京都大学, 薬学研究科(研究院), 准教授 (40273437)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAKURA Yoshinobu 京都大学, 大学院・薬学研究科, 教授 (30171432)
TAKAHASHI Yuki 京都大学, 大学院・薬学研究科, 助教 (00547870)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 核酸 / ナノ粒子 / DDS / CpG |
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| Research Abstract |
To increase the therapeutic potency of nucleic acid drugs, we r tried to develop polypod-like structured nucleic acid, or polypodna, and dendritic nano-sized DDS by connecting them. We found that polypodna with 3 to 8 pods can be developed and these DNA nanostructures are efficiently taken up by immune cells depending on pod number. Furthermore, we succeeded in constructing DNA dendrimer by connecting polypodnas through their adhesive terminal ends. Cytokine release by immunostimulatory CpG DNA has been greatly increased by the development of such DNA nanostructures. Thus, we have shown that this approach can be useful for increasing the potency of nucleic acid drugs.
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[Journal Article] Injectable, self-gelling, biodegradable, and immunomodulatory DNA hydrogel for antigen delivery2014
Author(s)
Nishikawa M, Ogawa K, Umeki Y, Mohri K, Kawasaki Y, Watanabe H, Takahashi N, Kusuki E, Takahashi R, Takahashi Y, Takakura Y
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Journal Title
Journal of Controlled Release
Volume: 180
Pages: 25-32
DOI
Peer Reviewed
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[Journal Article] Design and development of nanosized DNA assemblies in polypod-like structures as efficient vehicles for immunostimulatory CpG motifs to immune cells2012
Author(s)
Mohri K, Nishikawa M, Takahashi N, Shiomi T, Matsuoka N, Ogawa K, Endo M, Hidaka K, Sugiyama H, Takahashi Y, Takakura Y
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Journal Title
ACS Nano
Volume: 6
Pages: 5931-5940
DOI
Peer Reviewed
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[Presentation] 多足型核酸構造体-ポリポドナ-の設計と構造・機能解析2012
Author(s)
西川元也, 毛利浩太, 高橋夏樹, 楠木絵理, 塩見朋紀, 小川耕平, 梅木佑夏, 遠藤政幸, 杉山 弘, 渡辺 宏, 高橋有己, 高倉喜信
Organizer
アンチセンス・遺伝子・デリバリーシンポジウム2012
Place of Presentation
仙台
Year and Date
20120924-26
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