2013 Fiscal Year Final Research Report
Molecular mechanism for roles of chondroitin sulfate in tumor metastasis
| Project/Area Number |
23390016
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Hokkaido University |
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Principal Investigator |
SUGAHARA Kazuyuki 北海道大学, 先端生命科学研究科(研究院), 学術研究員 (60154449)
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| Co-Investigator(Kenkyū-buntansha) |
YAMADA Shuhei 北海道大学, 大学院・先端生命科学研究院, 准教授 (70240017)
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| Co-Investigator(Renkei-kenkyūsha) |
MIZUMOTO Shuji 名城大学, 薬学部, 助教 (40443973)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 癌 / 転移 / コンドロイチン硫酸 / ヘパラン硫酸 / RAGE / ルイス肺癌細胞 / グリコサミノグリカン / プロテオミクス |
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| Research Abstract |
Previously, we have demonstrated that Lewis lung carcinoma (LLC)-derived tumor cell line with high metastatic potential shows a higher proportion of E-disaccharide units, GlcUA-GalNAc(4, 6-O-disulfate), in CS chains, which are recognized by phage display antibody. Interestingly, the antibody strongly inhibited the pulmonary metastasis. In this research, to elucidate the molecular mechanism for tumor metastasis involved in the CS chains. The receptor molecule for CS chains containing E-disaccharides, Receptor for Advanced Glycation End-products (RAGE), was identified by proteomics approach. Furthermore, the colonization of the lungs by LLC cells was effectively inhibited by an anti-RAGE antibody. These results provide the clear evidence that RAGE is at least one of the critical receptors for CS chains expressed at the tumor cell surface and involved in experimental lung metastasis, and that CS/HS and RAGE are potential molecular targets in the treatment of pulmonary metastasis.
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Research Products
(41 results)
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[Journal Article] Unique heparan sulfate from shrimp heads exhibits a strong inhibitory effect on infections by dengue virus and Japanese encephalitis virus2011
Author(s)
Jiancheng Chen, Shuhei Yamada, Yoshiki Hama, Ajaya Kumar Shetty, Takanari Kobayashi, Hiroshi Oda, Kosuke Seiki, Eunmi Kim, Takashi Kimura, Naonori Takahashid, Kazuya Hidari, Takashi Suzuki, Yasuo Suzuki, Kazuyuki Sugahara
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Journal Title
Biochemical and Biophysical Research Communications
Volume: 412(1)
Pages: 136-142
Peer Reviewed
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[Journal Article] Faulty initiation of proteoglycan synthesis causes cardiac and joint defects2011
Author(s)
Sevjidmaa Baasanjav, Lihadh Al-Gazali, Taishi Hashiguchi, Shuji Mizumoto, Bjoern Fischer, Denise Horn, Dominik Seelow, Bassam R. Ali, Samir A.A. Aziz, Ruth Langer, Ahmed A.H. Saleh, Christian Becker, Gudrun Nürnberg, Vincent Cantagrel, Joseph G. Gleeson, Delphine Gomez, Jean-Baptiste Michel, Sigmar Stricker, Tom H. Lindner, Peter Nürnberg, Kazuyuki Sugahara, Stefan Mundlos, Katrin Hoffmann
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Journal Title
American Journal of Human Genetics
Volume: 89(1)
Pages: 15-27
Peer Reviewed
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[Presentation] グルクロン酸転移酵素-I の変異によって引き起こされる新規ラーセン様症候群2012
Author(s)
水本秀二, Sevjidmaa Baasanjav, Lihadh Al-Gazali, 橋口太志, Bjoern Fischer, Denise Horn, Dominik Seelow, Bassam R. Ali, Samir A.A. Aziz, Ruth Langer, Ahmed A.H. Saleh, Christian Becker, Gudrun Nürnberg, Vincent Cantagrel, Joseph G. Gleeson, Delphine Gomez, Jean-Baptiste Michel, Sigmar Stricker, Tom H. Lindner, Peter Nürnberg, Stefan Mundlos, Katrin Hoffmann, 菅原一幸
Organizer
日本薬学会第132年会
Place of Presentation
北海道大学(札幌市)
Year and Date
2012-03-29
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[Presentation] エビ頭部に由来するヘパラン硫酸の構造と生物活性2011
Author(s)
羽磨紀城, 陳建成, Ajaya Kumar Shetty, 小林孝成, 織田浩司, 清木興介, Eunmi Kim, 木村享史, 菅原一幸, 山田修平
Organizer
第30回日本糖質学会年会
Place of Presentation
長岡リリックホール(長岡市)
Year and Date
20110711-13
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[Presentation] Loss-of-function mutation of glucuronyltransferase-I causes Larsen-like syndrome2011
Author(s)
S. Baasanjav, L. Al-Gazali, T. Hashiguchi, ○S. Mizumoto, B. Fischer, D. Horn, D. Seelow, B. R. Ali, S.A.A. Aziz, R. Langer, A. A.H. Saleh, C. Becker, G. Nürnberg, V. Cantagrel, J. G. Gleeson, D. Gomez, J.-B. Michel, S. Stricker, T. H. Lindner, P. Nürnberg, K. Sugahara, S. Mundlos, K. Hoffmann
Organizer
7th International Conference on Proteoglycans
Place of Presentation
Q STATION (Australia)
Year and Date
2011-10-18
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[Presentation] A deficiency of glycosaminoglycans causes cardiac and joint disorders2011
Author(s)
Sevjidmaa Baasanjav, Lihadh Al-Gazali, Taishi Hashiguchi, ○ Shuji Mizumoto, Bjoern Fischer, Denise Horn, Dominik Seelow, Bassam R. Ali, Samir A.A. Aziz, Ruth Langer, Ahmed A.H. Saleh, Christian Becker, Gudrun Nürnberg, Vincent Cantagrel, Joseph G. Gleeson, Delphine Gomez, Jean-Baptiste Michel, Sigmar Stricker, Tom H. Lindner, Peter Nürnberg, Kazuyuki Sugahara, Stefan Mundlos, Katrin Hoffmann
Organizer
第84回日本生化学会大会
Place of Presentation
国立京都国際会館(京都市)
Year and Date
2011-09-24
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[Presentation] エビ頭部由来へパラン硫酸の構造と機能の研究2011
Author(s)
陳建成, 羽磨紀城, Ajaya Kumar Shetty, 小林孝成, 織田浩司, 清木興介, 木村享史, 高橋尚徳, 左一八, 鈴木隆, 鈴木康夫, ○山田修平, 菅原一幸
Organizer
日本薬学会北海道支部第136回例会
Place of Presentation
札幌コンベンションセンター(北海道)
Year and Date
2011-05-21
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