2013 Fiscal Year Final Research Report
Epigenetic regulation of retinoic acid-producing capacity of dendritic cells depending on their lineages
| Project/Area Number |
23390023
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Tokushima Bunri University |
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Principal Investigator |
IWATA Makoto 徳島文理大学, 薬学部, 教授 (50160122)
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| Co-Investigator(Kenkyū-buntansha) |
SONG Si-young 徳島文理大学, 神経科学研究所, 教授 (00399693)
KADOWAKI Norimitsu 京都大学, 大学院・医学系研究科, 准教授 (60324620)
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| Co-Investigator(Renkei-kenkyūsha) |
OHOKA Yoshiharu 徳島文理大学, 香川薬学部, 准教授 (60303971)
TAKEUCHI Hajime 徳島文理大学, 香川薬学部, 准教授 (00421298)
NAKATSUMA Aya 徳島文理大学, 香川薬学部, 助教 (30446075)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 免疫学 / ビタミンA / レチノイン酸 / 樹状細胞 / T細胞 / DNAメチル化 |
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| Research Abstract |
Retinoic acid (RA)-producing dendritic cells (DC) are present in the gut-related tissue, and play important roles in the gut immunity. ALDH1A2 is the key enzyme for producing RA in these DC. The Aldh1a2 promoter region was GC-rich, and contained an Sp1-binding site and an RA response element (RARE) half-site near the TATA box. Among various animal species, the sequences of this region were well conserved. Sp1 and the RA receptor (RAR)/retinoid X receptor (RXR) heterodimer mutually enhanced their binding to these sites and cooperatively enhanced the promoter activity in the presence of RA. Accordingly, RA is an essential co-factor for inducing ALDH1A2 expression in DC. DNA methylation in the Aldh1a2 promoter region inhibited its activation. However, in normal DC subsets and other immune cells, the epigenetic regulation by Sp1 or histone acetylation but not DNA methylation appeared to participate in the regulation of RALDH2 gene expression.
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[Journal Article] Human CD1c^+ myeloid dendritic cells acquire a high level of retinoic acid-producing capacity in response to vitamin D_32013
Author(s)
Sato T, Kitawaki T, Fujita H, Iwata M, Iyoda T, Inaba K, Ohteki T, Hasegawa S, Kawada K, Sakai Y, Ikeuchi H, Nakase H, Niwa A, Takaori-Kondo A, and Kadowaki N
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Journal Title
J Immunol
Volume: 191(6)
Pages: 3152-3160
DOI
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[Journal Article] A phase I/IIa clinical trial of immunotherapy for elderly patients with acute myeloid leukemia using dendritic cells co-pulsed with WT1 peptide and zoledronate2011
Author(s)
Kitawaki T, Kadowaki N, Fukunaga K, Kasai Y, Maekawa T, Ohmori K, Kondo T, Maekawa R, Takahara M, Nieda M, Kuzushima K, Ishikawa T, Uchiyama T
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Journal Title
Br J Haematol
Volume: 153
Pages: 796-799
DOI
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[Journal Article] Cross-priming of CD8^+ T cells in vivo by dendritic cells pulsed with autologous apoptotic leukemic cells in immunotherapy for elderly patients with acute myeloid leukemia2011
Author(s)
Kitawaki T, Kadowaki N, Fukunaga K, Kasai Y, Maekawa T, Ohmori K, Itoh T, Shimizu A, Kuzushima K, Kondo T, Ishikawa T, Uchiyama T
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Journal Title
Exp Hematol
Volume: 39
Pages: 424-433
DOI
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[Presentation] ビタミンD 刺激によってレチノイン酸を高産生するヒト樹状細胞の同定2013
Author(s)
佐藤貴之, 北脇年雄, 藤田晴之, 岩田誠, 伊豫田智典, 稲葉カヨ, 樗木俊聡, 長谷川傑, 河田健二, 坂井義治, 池内浩基, 高折晃史, 門脇則光
Organizer
第17回日本がん免疫学会総会
Place of Presentation
宇部
Year and Date
2013-07-04
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