2013 Fiscal Year Final Research Report
Generation of Monkey/Mouse Chimera with Monkey Liver by Interspecific Blastocyst Injection of Monkey induced Pluripotent Stem Cells
| Project/Area Number |
23390036
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Medical pharmacy
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| Research Institution | Nagoya City University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OHMORI Shigeru 信州大学, 医学部附属病院, 教授 (70169069)
NARIMATSU Shizuo 岡山大学, 医歯薬学総合研究科, 教授 (20113037)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 胚盤胞補完法 / サルiPS細胞 / 異種動物間キメラ / ラットiPS細胞 / カニクイザル / マーモセット / ラット / tk-NOGマウス |
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| Research Abstract |
Monkey iPS cell lines were established from dermal fibroblasts by transforming of reprogramming factors. The differentiation ability into triploblastic cells of the iPS cells in vitro was determined by floating cultivation of embryoid bodies. To examine the potential for xenogenic approaches in blastocyst complementation, we injected rat iPS/ES cells into mouse blastocysts generating interspecific chimeras. The interspecific chimeras between mouse and rat using a blastocyst injection technique with rat iPS/ES cells were generated. These data provide basic knowledge of principle for interspecific blastocyst complementation and for generation in vivo of organs derived from monkey iPS/ES cells using a xenogenic environment.
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[Journal Article] Selective culture method for hepatocyte-like cells differentiated from human induced pluripotent stem cells2014
Author(s)
Kondo Y, Yoshihashi S, Mimori K, Ogihara R, Kanehama Y, Maki Y, Enosawa S, Kurose K, Iwao T, Nakamura K, Matsunaga T
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Journal Title
DOI
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[Journal Article] Establishment and directed differentiation of induced pluripotent stem cells from glycogen storage disease type Ib patient2013
Author(s)
Satoh D, Maeda T, Ito T, Nakajima Y, Ohte M, Ukai A, Nakamura K, Enosawa S, Toyota M, Miyagawa Y, Okita H, Kiyokawa N, Akutsu H, Umezawa A, Matsunaga T
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Journal Title
Genes Cells
Volume: 18
Pages: 1053-1069
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[Journal Article] An efficient method for differentiation of human induced pluripotent stem cells into hepatocyte-like cells retaining drug metabolizing activity2013
Author(s)
Kondo Y, Iwao T, Nakamura K, Sasaki T, Takahashi S, Kamada N, Matsubara T, Gonzalez FJ, Akutsu H, Miyagawa Y, Okita H, Kiyokawa N, Toyoda M, Umezawa A, Nagata K, Matsunaga T, Ohmori S
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Journal Title
DOI
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[Presentation] Pharmacokinetic function of hepatocyte-like cells differentiated from human iPS cells2013
Author(s)
Kondo Y, Iwao T, Ogihara R, Sasaki T, Nagata K, Kurose K, Horikawa T, Niwa T, Yamaori S, Ohmori S, Nakamura K Matsunaga T
Organizer
日本薬物動態学会第28回年会
Place of Presentation
東京
Year and Date
20131000
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[Presentation] ヒト人工多能性幹細胞の肝細胞への分化における変法L-15 培地及び血清の効果2013
Author(s)
松永民秀, 近藤祐樹, 荻原留理, 岩尾岳洋, 永田 清, 黒瀬光一, 堀川隆司, 丹羽卓朗, 山折 大, 大森 栄, 中村克徳
Organizer
フォーラム2013 : 衛生薬学・環境トキシコロジー
Place of Presentation
福岡
Year and Date
20130900
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[Presentation] 低分子化合物はヒト人工多能性幹細胞から肝細胞への分化を促進する2012
Author(s)
近藤祐樹, 岩尾岳洋, 吉橋幸美, 三森佳代, 杉山留理, 佐々木崇光, 永田 清, 黒瀬光一, 丹羽卓朗, 山折 大, 大森 栄, 中村克徳, 松永民秀
Organizer
日本薬物動態学会第27回年会
Place of Presentation
東京
Year and Date
20121100
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