2013 Fiscal Year Final Research Report
Genetic study of molecular characteristic of autophagy-related proteins LC3A and LC3B
| Project/Area Number |
23390041
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KOIKE Masato 順天堂大学, 医学部, 先任准教授 (80347210)
SASAKI Mitsuho 順天堂大学, 医学部, 助教 (20432536)
SUNABORI Takehiko (00407115)
SUZUKI Chigure 順天堂大学, 医学部, 助教 (40536629)
SHIBATA Masahiro 新潟大学, 医歯学総合研究科, 准教授 (10343253)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | LC3 / オートファジー / 脂肪滴形成 |
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| Research Abstract |
The roles of autophagy-related proteins LC3A and LC3B largely remains unknown, although LC3 are used as a marker protein for autophagosome formation. To further understand their roles, single and double knockout (KO) mice of LC3A and LC3B were produced. These KO mice were born normally and grown-up to be capable of reproduction. Protein expression of the GABARAP family of proteins did not change between wild-type and LC3A/B-deficient mouse body tissues such as brain, liver, kidney and heart, indicating they maintain basic autophagy. Hippocampal pyramidal neurons of neonatal brains deficient in LC3A/B were resistant to hypoxic-ischemic brain injury. The result strongly suggests the presence of autophagic neuron death in the hippocampal pyramidal layer of mouse brains.
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