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2013 Fiscal Year Final Research Report

Study on structure and function of mitochondria Na-Ca exchange(NCLX)

Research Project

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Project/Area Number 23390042
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General physiology
Research InstitutionUniversity of Fukui (2013)
Kyoto University (2011-2012)

Principal Investigator

MATSUOKA Satoshi  福井大学, 医学部, 教授 (00263096)

Co-Investigator(Kenkyū-buntansha) TAKEUCHI Ayako  福井大学, 医学部, 特命助教 (00378704)
Co-Investigator(Renkei-kenkyūsha) KIM Bongju  徳島大学, 疾患プロテオゲノム研究センター, 助教 (80511823)
Project Period (FY) 2011-04-01 – 2014-03-31
Keywordsトランスポーター / ミトコンドリア / カルシウム / 心筋細胞 / リンパ球 / シミュレーション
Research Abstract

In B lymphocyte cell lines (A20 and DT 40), it was demonstrated that mitochondrial Na/Ca exchanger (NCLX) is associated with mitochondrial Ca extrusion, modulation of endoplasmic reticulum Ca content, endoplasmic reticulum Ca release upon BCR-stimulation, store-operated Ca entry and chemotaxis. In cardiomyocyte cell line (HL-1), it was also demonstrated NCLX extrudes mitochondrial Ca and modulates sarcoplasmic reticulum Ca content. Additionally, it was found that NCLX modulates spontaneous beating frequency. These results were well reproduced by mathematical models of B lymphocyte, HL-1 cell and sinoatrial node cell. Taken together, it was suggested that NCLX act as a Ca provider from mitochondria to sarco- and endo-plasmic reticulum.

  • Research Products

    (36 results)

All 2014 2013 2012 2011 Other

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (22 results) (of which Invited: 4 results) Book (2 results) Remarks (2 results)

  • [Journal Article] Identification of chemicals inducing cardiomyocyte proliferation in developmental stage-specific manner with pluripotent stem cells2013

    • Author(s)
      Uosaki H, Magadum A, Seo K, Fukushima H, Takeuchi A, Nakagawa Y, Moyes KW, Narazaki G, Kuwahara K, Laflamme M, Matsuoka S, Nakatsuji N, Nakao K, Kwon C, Kass DA, Engel FB, Yamashita JK
    • Journal Title

      Circ Cardiovasc Genet

      Volume: 6(6) Pages: 624-33

    • DOI

      10.1161/CIRCGENETICS.113.000330

    • Peer Reviewed
  • [Journal Article] The mitochondrial Na^+-Ca^<2+> exchanger, NCLX, regulates automaticity of HL-1 cardiomyocytes2013

    • Author(s)
      Takeuchi A, Kim B, Matsuoka S
    • Journal Title

      Sci Rep

      Volume: 3 Pages: 2766

    • DOI

      10.1038/srep02766

    • Peer Reviewed
  • [Journal Article] Basophils are required for the induction of Th2 immunity to haptens and peptide antigens2013

    • Author(s)
      Otsuka A, Nakajima S, Kubo M, Egawa G, Honda T, Kitoh A, Nomura T, Hanakawa S, Sagita Moniaga C, Kim B, Matsuoka S, Watanabe T, Miyachi Y, Kabashima K
    • Journal Title

      Nat Commun

      Volume: 4 Pages: 1738

    • DOI

      10.1038/ncomms2740

    • Peer Reviewed
  • [Journal Article] Mitochondria Na^+-Ca^<2+> exchange in cardiomyocytes and lymphocytes2013

    • Author(s)
      Kim B, Takeuchi A, Koga O, Hikida M, Matsuoka S
    • Journal Title

      Adv Exp Med Biol

      Volume: 961 Pages: 193-201

    • DOI

      10.1007/978-1-4614-4756-6-16

    • Peer Reviewed
  • [Journal Article] NHE-1 blockade reversed changes in calcium transient in myocardial slices from isoproterenol-induced hypertrophied rat left ventricle2012

    • Author(s)
      Hattori H, Takeshita D, Takeuchi A, Kim B, Shibata M, Matsuoka S, Obata K, Mitsuyama S, Zhang GX, Takaki M
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 419(2) Pages: 431-5

    • DOI

      10.1016/j.bbrc.2012.02.041

    • Peer Reviewed
  • [Journal Article] Pivotal role of mitochondrial Na^+-Ca^<2+> exchange in antigen receptor mediated Ca^<2+> signalling in DT40 and A20 B lymphocytes2012

    • Author(s)
      Kim B, Takeuchi A, Koga O, Hikida M, Matsuoka S
    • Journal Title

      J Physiol

      Volume: 590(3) Pages: 459-74

    • DOI

      10.1113/jphysiol.2011.222927

    • Peer Reviewed
  • [Journal Article] Impairment of Ubiquitin-Proteasome System by E334K cMyBPC Modifies Channel Proteins, Leading to Electrophysiological Dysfunction2011

    • Author(s)
      Bahrudin U, Morikawa K, Takeuchi A, Kurata Y, Miake J, Mizuta E, Adachi K, Higaki K, Yamamoto Y, Shirayoshi Y, Yoshida A, Kato M, Yamamoto K, Nanba E, Morisaki H, Morisaki T, Matsuoka S, Ninomiya H, Hisatome I
    • Journal Title

      J Mol Biol

      Volume: 413(4) Pages: 857-78

    • DOI

      10.1016/j.jmb.2011.09.006

    • Peer Reviewed
  • [Journal Article] Requirement of Interaction between Mast Cells and Skin Dendritic Cells to Establish Contact Hypersensitivity2011

    • Author(s)
      Otsuka A, Kubo M, Honda T, Egawa G, Nakajima S, Tanizaki H, Kim B, Matsuoka S, Watanabe T, Nakae S, Miyachi Y, Kabashima K
    • Journal Title

      PLoS One

      Volume: 6(9) Pages: e25538

    • DOI

      10.1371/journal.pone.0025538

    • Peer Reviewed
  • [Journal Article] Minimum Information about a Cardiac Electrophysiology Experiment (MICEE) : Standardised reporting for model reproducibility, interoperability, and data sharing2011

    • Author(s)
      Quinn TA, Granite S, Allessie MA, Antzelevitch C, Bollensdorff C, Bub G, Burton RA, Cerbai E, Chen PS, Delmar M, Difrancesco D, Earm YE, Efimov IR, Egger M, Entcheva E, Fink M, Fischmeister R, Franz MR, Garny A, Giles WR, Hannes T, Harding SE, Hunter PJ, Iribe G, Jalife J, Johnson CR, Kass RS, Kodama I, Koren G, Lord P, Markhasin VS, Matsuoka S, McCulloch AD, Mirams GR, Morley GE, Nattel S, Noble D, Olesen SP, Panfilov AV, Trayanova NA, Ravens U, Richard S, Rosenbaum DS, Rudy Y, Sachs F, Sachse FB, Saint DA, Schotten U, Solovyova O, Taggart P, Tung L, Varró A, Volders PG, Wang K, Weiss JN, Wettwer E, White E, Wilders R, Winslow RL, Kohl P
    • Journal Title

      Prog Biophys Mol Biol

      Volume: 107(1) Pages: 4-10

    • DOI

      10.1016/j.pbiomolbio.2011.07.001

    • Peer Reviewed
  • [Journal Article] Efficient and Scalable Purification of Cardiomyocytes from Human Embryonic and Induced Pluripotent Stem Cells by VCAM1 Surface Expression2011

    • Author(s)
      Uosaki H, Fukushima H, Takeuchi A, Matsuoka S, Nakatsuji N, Yamanaka S, Yamashita JK
    • Journal Title

      PLoS One

      Volume: 6(8) Pages: e23657

    • DOI

      10.1371/journal.pone.0023657

    • Peer Reviewed
  • [Presentation] リンパ球Ca 応答の数理モデル解析2014

    • Author(s)
      松岡 達, 金 鳳柱, 竹内 綾子, 古賀 織絵, 疋田 正喜
    • Organizer
      第91回日本生理学会大会シンポジウム "数理生理学の新たな展開"
    • Place of Presentation
      鹿児島市(鹿児島大学)
    • Year and Date
      20140316-18
  • [Presentation] ミトコンドリアNa-Ca 交換体NCLX を介した心筋ミトコンドリア-筋小胞体Ca クロストークと自動能制御2014

    • Author(s)
      竹内 綾子, 松岡 達
    • Organizer
      第91回日本生理学会大会シンポジウム"(興奮性細胞におけるオルガネラ特異的なCa^<2+>シグナリングと生体応答"
    • Place of Presentation
      鹿児島市(鹿児島大学)
    • Year and Date
      20140316-18
  • [Presentation] ミトコンドリア-(筋)小胞体Ca^<2+>クロストークと細胞機能連関2014

    • Author(s)
      竹内 綾子, 金 鳳柱, 松岡 達
    • Organizer
      第91回日本生理学会大会
    • Place of Presentation
      鹿児島市(鹿児島大学)
    • Year and Date
      20140316-18
  • [Presentation] 細胞容積調節におけるチャネル・トランスポーターの寄与~包括的心筋細胞数理モデルと細胞生理実験によるイオン・水動態の解明~2014

    • Author(s)
      竹内綾子, 松岡達
    • Organizer
      水シグナル研究会「水シグナリングの分子動態から病態へ」
    • Place of Presentation
      福井市 アオッサ
    • Year and Date
      20140305-06
  • [Presentation] Mitochondria-Sarcoplasmic Reticulum Ca^<2+> Communication on the Rhythmicity of Sinoatrial Node Cells2013

    • Author(s)
      Matsuoka S, Takeuchi A
    • Organizer
      Cardiac Physiome Workshop 2013
    • Place of Presentation
      Bar Harbor Regency, Bar Harbor, ME
    • Year and Date
      20131017-19
    • Invited
  • [Presentation] Simulation analyses of the role of NCLX, a mitochondrial Na^+-Ca^<2+> exchanger, on the regulation of automaticity in sinoatrial node cells2013

    • Author(s)
      Takeuchi A, Matsuoka S
    • Organizer
      Cardiac Physiome Workshop 2013
    • Place of Presentation
      Bar Harbor Regency, Bar Harbor, ME. USA
    • Year and Date
      20131017-19
  • [Presentation] B 細胞の走化性を調節するミトコンドリアNCX2013

    • Author(s)
      金 鳳柱, 竹内 綾子, 松岡 達
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      東京
    • Year and Date
      20130327-29
  • [Presentation] ミトコンドリアNCX(NCLX)による心自動能制御-数理モデル解析による考察2013

    • Author(s)
      竹内綾子, 金鳳柱, 松岡達
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      東京
    • Year and Date
      20130327-29
  • [Presentation] Role of mitochondrial NCX on CXCL12-induced chemotaxis in A20 B lymphocytes2013

    • Author(s)
      Kim B, Takeuchi A, Matsuoka S
    • Organizer
      57th Annual Meeting of Biophysical Society
    • Place of Presentation
      Philadelphia, USA
    • Year and Date
      20130202-06
  • [Presentation] Prolongation of beating rate caused by reduction of NCLX, a mitochondrial Na^+-Ca^<2+> exchanger, in HL-1 cardiomyocytes2013

    • Author(s)
      Takeuchi A, Kim B, Matsuoka S
    • Organizer
      57th Annual Meeting of Biophysical Society
    • Place of Presentation
      Philadelphia, USA
    • Year and Date
      20130202-06
  • [Presentation] Contribution of Mitochondrial Na^+-Ca^<2+> Exchanger, NCLX, in Making the Cardiac Rhythmicity. A Combination Study of Experiments and Simulations2013

    • Author(s)
      Takeuchi A, Kim B, Matsuoka S
    • Organizer
      The 2nd HD Physiology International Symposium : Multi-Level Systems Biology
    • Place of Presentation
      東京丸の内MY PLAZA
    • Year and Date
      2013-06-28
  • [Presentation] Fine-Tuning of Cardiac Automaticity by Mitochondria Na^+-Ca^<2+> Exchange (NCLX)2012

    • Author(s)
      Satoshi Matsuoka, Ayako Takeuchi, Bongju Kim
    • Organizer
      2012 Cardiac Physiome Workshop
    • Place of Presentation
      San Diego USA
    • Year and Date
      20121030-1102
    • Invited
  • [Presentation] Mechanism for NCLX reduction-mediated slowing of cardiomyocyte beating rate2012

    • Author(s)
      Takeuchi A, Kim B, Matsuoka S
    • Organizer
      2012 Cardiac Physiome Workshop
    • Place of Presentation
      San Diego USA
    • Year and Date
      20121030-1102
  • [Presentation] Regulation of cardiomyocyte function via mitochondrial NCX (NCLX)2012

    • Author(s)
      Takeuchi A, Kim B and Matsuoka S
    • Organizer
      シンポジウム「心筋のE-C カップリング : 心機能と病態を制御する新しいCa^<2+>シグナル調節分子」 第89回日本生理学会大会
    • Place of Presentation
      松本市(松本文化会館)
    • Year and Date
      20120329-31
  • [Presentation] Role of mitochondrial NCX on CXCL12-induced chemotaxis in B lymphocytes2012

    • Author(s)
      Kim B, Takeuchi A and Matsuoka S
    • Organizer
      第89回日本生理学会大会
    • Place of Presentation
      松本市(松本文化会館)
    • Year and Date
      20120329-31
  • [Presentation] Na^+/H^+ exchanger-1 blocker treatment reversed changes in calcium transient in myocardial slices from isoproterenol-induced hypertrophied rat left ventricles2012

    • Author(s)
      Takeshita D, Hattoria H, Matsuoka S, Shibata M, Kim B, Takeuchi A, Zhang G, Obata K, and Takaki M
    • Organizer
      第89回日本生理学会大会
    • Place of Presentation
      松本市(松本文化会館)
    • Year and Date
      20120329-31
  • [Presentation] Role of mitochondrial Na-Ca exchange on BCR-mediated Ca^<2+> signalling in B lymphocytes2012

    • Author(s)
      Kim B, Takeuchi A, Koga O, Hikida M, and Matsuoka S
    • Organizer
      56th Annual Meeting of Biophysical Society
    • Place of Presentation
      San Diego, California, USA
    • Year and Date
      20120225-29
  • [Presentation] Role of mitochondrial Na-Ca exchange on B Cell Receptor-mediated Ca^<2+> signaling in DT40 and A20 B lymphocytes2011

    • Author(s)
      Bongju Kim, Ayako Takeuchi, Orie Koga, Masaki Hikida, and Satoshi Matsuoka
    • Organizer
      日光シンポジウム : 膜輸送体研究の前衛を目指して
    • Place of Presentation
      日光市(日光東照宮晃陽苑)
    • Year and Date
      20111217-18
  • [Presentation] Roles of mitochondria NCX (NCLX) on antigen-receptor mediated Ca^<2+> signaling in B lymphocytes2011

    • Author(s)
      Satoshi Matsuoka, Bongju Kim, Ayako Takeuchi, Masaki Hikida
    • Organizer
      6th International Conference on Na^+/Ca^<2+> Exchange 2011
    • Place of Presentation
      Lacco Ameno, Ischia Island, Naples, Italy
    • Year and Date
      20111001-05
    • Invited
  • [Presentation] Functional characterization of a mitochondrial Na/Ca exchanger (NCLX) in cardiac cell line, HL-12011

    • Author(s)
      Takeuchi A, Kim B, Matsuoka S
    • Organizer
      6th International Conference on Na^+/Ca^<2+> Exchange 2011
    • Place of Presentation
      Lacco Ameno, Ischia Island, Naples, Italy
    • Year and Date
      20111001-05
  • [Presentation] Mitochondrial Na/Ca exchange in excitable and non-excitable cells2011

    • Author(s)
      Satoshi Matsuoka, Bongju Kim, Ayako Takeuchi, Masaki Hikida
    • Organizer
      4th Cardiac Physiome Conference
    • Place of Presentation
      Merton College, Oxford UK
    • Year and Date
      2011-07-08
    • Invited
  • [Presentation] Simulation study of Ca^<2+>-dependent mitochondrial regulation in cardiac excitation-contraction coupling2011

    • Author(s)
      Ayako Takeuchi, Ryuta Saito, Yukiko Himeno, Akinori Noma, and Satoshi Matsuoka
    • Organizer
      4th Cardiac Physiome Conference
    • Place of Presentation
      Merton College, Oxford UK
    • Year and Date
      2011-07-08
  • [Book] 心室筋細胞興奮収縮連関のシミュレーション実習 新訂・生理学実習書2013

    • Author(s)
      竹内綾子・松岡達
    • Total Pages
      310
    • Publisher
      南江堂
  • [Book] Giant patch and macro patch. In Patch clamp techniques. From beginning to advanced protocols. Edited by Okada Y2012

    • Author(s)
      Matsuoka S, Takeuchi A
    • Total Pages
      439
    • Publisher
      Springer
  • [Remarks]

    • URL

      http://isphysio.med.u-fukui.ac.jp/index.html

  • [Remarks]

    • URL

      http://seiri2.med.lab.u-fukui.ac.jp/

URL: 

Published: 2015-06-25  

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